November 2012
Recent advances and application of doubled haploids in wheat breeding
Genetic improvement to develop varieties with high yield potential and resistance/tolerance to abiotic and biotic stresses with acceptable end use qualities is the most viable and environment friendly option to increase wheat yield in a sustainable fashion. In vitro haploid production followed by chromosome doubling greatly enhances the production of complete homozygous wheat lines in a single generation...
November 2012
Isolation and characterization of sixty sequences of cot-1 DNA from the Asiatic black bear, Ursus thibetanus
The Asiatic black bear (Ursus thibetanus) is a class II protected species in China whose genome is not well characterized. All mammalian genomes have a high proportion of repetitive sequences; thus, studying the repetitive sequences of the Asiatic black bear can provide new insight into the organization of its genome. It is well known that cot-1 DNA is enriched for repetitive DNA elements with high and...
November 2012
Molecular marker analysis of ‘Shatangju’ and ‘Wuzishatangju’ mandarin (Citrus reticulata Blanco)
‘Wuzishatangju’(Citrus reticulata Blanco) is an excellent cultivar derived from a bud sport of a seedy ‘Shatangju’ cultivar found in Guangdong Province in the 1980s. In this study, six molecular markers including random amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), simple sequence repeat (SSR), sequence-related amplified polymorphism (SRAP),...
November 2012
Molecular characterization of bacteria isolated from the Kingdom of Saudi Arabia and their uses against pathogenic fungi causing dermatological diseases
Random amplified polymorphic DNA (RAPD) assay and antimicrobial activities were used for the detection of genetic variations of antimicrobial isolates from the Kingdom Saudi of Arabia. Soil samples were collected from El-Madina El-Monawara, El–Taif, El-Jouf and El-Dammam for bacterial isolation. Bacterial isolates were evaluated for their antimicrobial activities against pathogenic fungi which cause...
November 2012
Barley breeding for quality improvement in Tunisia
This study was initiated to assess the effectiveness of three selection procedures applied in the early segregating generations of barley crosses for quality traits. The selection procedures were pedigree selection (PS), bulk selection (BS) and single seed descent selection (SSD). Selection was operated in F2’s crosses. Field trials were conducted with 19 F3 crosses of barley in two different...
November 2012
Effect of medium, explants, cytokinins and node position on in vitro shoot multiplication of Caralluma lasiantha (Wight) N.E.Br., an endemic and medicinally important plant
An efficient shoot multiplication protocol was developed for Caralluma lasiantha, an endemic medicinal plant belonging to the family Asclepiadaceae. Mature explants were cultured on Murashige and Skoog (MS), (1962) medium, Gamborg's B5 (B5) and woody plant medium (WPM) supplemented with 6-benzyladenine (BA), 8.87 mM, for shoot induction. The nodal explants exhibited maximum shoot sprouting frequency when...
November 2012
Chitinolytic activity of highly halotolerant Streptomyces tendae against Fusarium oxysporum PTK2
Fifteen (15) highly halotolerant Streptomyces isolates were isolated from saline soil of Teachers College garden in Riyadh city. Chitin nitrate agar medium containing 20% (w/v) NaCl was used for the isolation purpose. These chitinolytic Streptomycesisolates were purified and sub-cultured on chitin nitrate broth medium containing 20% NaCl (w/v). The dry weight and absorbance (585 nm) were...
November 2012
A rapid in vitro protocol for propagation of Piper aduncum and Piper hispidinervum, two species from Amazon region with multipurpose uses
The morphogenic potential of nodal explants of Piper aduncum and Piper hispidinervum (Piperaceae) was investigated and a protocol for rapid micropropagation is described. An experiment based on the saline formulation of Murashige and Skoog (MS) and Wood Plant medium (WPM) combined with different N6-benzylaminopurine (BAP) and 1-naphthalene acetic acid (NAA) concentrations was evaluated....
November 2012
Molecular cloning, expression and computational analysis of a water stress inducible copper-containing amine oxidase gene (CuAO) from tea plant [Camellia sinensis (L.) O. Kuntze]
Copper-containing amine oxidase (CuAO) is the enzyme known to play diversity of function in plant responses to environmental stresses through its reaction products. Here, for the first time we report full length cDNA encoding CuAO protein from a drought tolerant tea cultivar. It was found to be 785 bp long with a 70 bp 5ʹ-UTR, 193 bp 3ʹ-UTR, 522 bp mORF and a polyA adenylational signal. It codes for a...
November 2012
Antimicrobial activity of Psidium guajava Linn. stem extracts against methicillin-resistant Staphylococcus aureus
The antimicrobial activities of the water and methanolic extracts of Psidium guajavaLinn. stem bark were evaluated against eight methicillin-resistant Staphylococcus aureus (MRSA) isolates. The plant material was extracted and phytochemical analyses were performed by standard procedures. The agar diffusion method was employed for the assessment of the sensitivity of the extracts, while the...
November 2012
Comparison of growth performance, carcass characteristics and meat quality of Benin indigenous chickens and Label Rouge (T55×SA51)
A study on growth performance, carcass traits and meat quality was carried out on Savannah and Forest ecotype chicken of Benin, using Label Rouge (T55 X SA51) as a control genotype. All the animals were fed ad libitum with three diets (starter, growerand layer feed). They were individually weighed at hatching and during the growingstage, and the daily feed intake was...
November 2012
Effect of corn silage particle size and level of soybean oil on ruminal mat composition, distribution and consistency in Zel sheep
To determine the effects of two corn silage particle size (coarse particle with geometric mean of 5.83 ± 2.47 mm and fine particle with geometric mean of 4.74 ± 2.74 mm) and two levels of soybean oil (0 and 4% of DM) on ruminal matcomposition, distribution and consistency, four two years fistulated ruminant Zel ewes (BW = 34 ± 1.4 kg), fed with 50:50 roughage-concentrate diet, were...
November 2012
Species specific polymerase chain reaction (PCR) assay for identification of pig (Sus domesticus) meat
A highly specific single step polymerase chain reaction (PCR) is described for the detection of pig (Sus domesticus) meat. A PCR assay was successfully optimized for amplification of 629 and 322-bp DNA fragment extracted from pig meat using designed species-specific primer pairs based on mitochondrial D-loop and 12Sribosomal ribonucleic acid (rRNA) gene, respectively. The optimized PCR assay...
November 2012
Impact of bee pollen as feed supplements on the body weight of broiler Ross 308
This study was aimed at investigating the meat performance of broiler Ross 308 after the application of bee pollen extract in their diet. The experiment group was fed with bee pollen (400 mg.kg-1) added in the feed. After analysis, it was found that the female chicken group’s average live body weight were higher in control group (2246.60 g) than that of experimental group (2194.40 g)....
November 2012
Antimicrobial activities of essential oils from Southern Africa against selected bacterial and fungal organisms
In the present study, essential oils from four plants including Melissa officinalis, Mentha piperita, Pelargonium graveolens and Leucosidea sericea, traditionally used to treat infectious diseases were tested for antimicrobial activity against seven Gram-positive bacteria, eight Gram-negative bacteria and six yeast species (Candida spp. and Cryptococcus neoformans) using the agar diffusion...
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