African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12196

Table of Content: 8 March 2010; 9(10)

March 2010

Premunition in Plasmodium falciparum malaria

Malaria parasites have evolved to maintain a well-balanced relationship with their human hosts. This implies that they can partially escape from protective effector mechanisms of their hosts, but also that hosts can develop partial immunity to the parasite. This immunity requires repeated infections, takes years to develop and is usually of short duration. However, protective immunity to clinical malaria rather than...

Author(s): R. K. Obi, C. C. Okangba, F. C. Nwanebu, U. U. Ndubuisi and N. M. Orji

March 2010

Prospective of biodiesel production utilizing microalgae as the cell factories: A comprehensive discussion

Microalgae are sunlight-driven miniature factories that convert atmospheric CO2 to polar and neutral lipids which after esterification can be utilized as an alternative source of petroleum. Further, other metabolic products such as bioethanol and biohydrogen produced by algal cells are also being considered for the same purpose. Microaglae are more efficient than the conventional oleaginous plants in capturing...

Author(s): Narendra Mohan Verma, Shakti Mehrotra, Amitesh Shukla and Bhartendu Nath Mishra

March 2010

Application of polymerase chain reaction to differentiate between strains of Campylobacter jejuni and Campylobacter coli

A polymerase chain reaction (PCR) assay was used to identify and differentiate between strains of Campylobacter jejuni and Campylobacter coli. NineCampylobacter reference strains; C. jejuni NCTC 11168, C. jejuni NCTC 11322, C. jejuni NCTC...

Author(s): Bin Jasass, F. M. and Park, S. F.

March 2010

Genetic diversity of Pogonatherum paniceum (Lam.) Hack in Southwest China revealed by ISSR

Inter-simple sequence repeats markers were used to estimate the genetic diversity of Pogonatherum paniceum (Lam.) Hack. from Sichuan Province, Yunnan Province, Chongqing City and Guangxi Zhuang  Autonomous Region in China. 100 primers were carried out on 22 wild populations, 14 could produce highly reproducible inter-simple sequence repeats markers′ bands. Out of the 239 discernable DNA...

Author(s): Zeng-Bin Luo#, Guo-Qing Zhuang#, Dan-Wei Ma and Fang Chen

March 2010

Identification of RAPD marker for the White Backed Plant Hopper (WBPH) resistant gene in rice

The experimental material consisted of two parents Gurjari (white backed plant hopper resistant) and Jaya (white backed plant hopper susceptible) and their F2 progeny. The purpose of the study was the identification of RAPD (Random Amplified Polymorphic DNA) marker for white backed plant hopper (WBPH) resistant gene. The RAPD analysis was done group wise as well as combined for the bulk segregant analysis (BSA)....

Author(s): Akarsh Parihar, A. R. Pathak and Pratibha Parihar

March 2010

Identification of AFLP markers linked with cocoon weight genes in silkworm (Bombyx mori L.)

DNA markers used in assisting selection method is a safe method in breeding process, due to deletion of environmental conditions, and it is an important tool in preparing linkage map and QTLs mapping. In mulberry silkworm that is, foundation of world sericulture, its major production- economic characteristics are polygenic. In this study, we want to determine QTL(s) affecting cocoon weight trait by AFLP...

Author(s): S. Z. Mirhosseini, A. R. Bizhannia, B. Rabiei, M. Taeb and A. R. Siedavi

March 2010

Analyses of spatial variations of kenaf in experimental field

Preliminary investigations of experimental field usually involve collection of soil samples at widely spaced locations which are patchily or globally at variant spatially. This study was carried out to evaluate spatial variations in experimental fields using a split plot experiment distributed in a completely randomized design at Ikenne and Ilora between June and September 2006 (test crop was kenaf). The preliminary...

Author(s): Dauda, T. O., Asiribo, O. E., Adekeye, K. S. and Agbaje, G. A.

March 2010

Effect of sowing depth and mulch application on emergence and growth of shea butter tree seedlings (Vitellaria Paradoxa C. F. Gaertn.)

Shea butter tree seeds from three sources (Makurdi, Akwanga and Kano), were sown at five depths (2, 4, 6, 8 and 10 cm) under mulch and no-mulch conditions at Makurdi in 2006. The aim was to determine the effect of seed source, sowing depth and mulching status on seedling emergence and growth. Factorial combinations of the treatments were arranged in randomized complete block design (RCBD) replicated three times....

Author(s): F. D. Ugese, K. P. Baiyeri and B. N. Mbah

March 2010

Pigment identification and antioxidant properties of red dragon fruit (Hylocereus polyrhizus)

The aim of this study was to identify pigments present in the red dragon fruit (Hylocereus polyrhizus) and to further investigate the antioxidant properties in this fruit. Pigment was identified using the High Performance Liquid Chromatography (HPLC) and results confirmed the presence of betanin in sample at a retention time of 11.5 min which corresponded to the retention time of the betanin...

Author(s): O. P. S. Rebecca, A. N. Boyce and S. Chandran

March 2010

Epidermal leaf characteristics and seasonal changes of net photosynthesis of five Populus

Net photosynthesis (PN) and leaf epidermal characteristics of five poplars; 72-31 (Populus alba x Populus glandulosa), Eco28 (Populus euramericana), Suwon (Populus koreana x Populus nigra var. italica), Dorskamp (Populus deltoides x P. nigra), and 62-2 (P. nigra x Populus maximowiczii) were investigated to come up with parameters for comparison. The...

Author(s): Su Young Woo

March 2010

Cloning of phenazine carboxylic acid genes of Fusarium fujikuroi antagonists bacteria

Bakanae disease caused by Fusarium fujikuroi is an important diseases on rice.Pseudomonas fluorescens produces the broad-spectrum antibiotic phenazine-carboxylic acid (PCA), which is active against a variety of fungal root pathogens. In this study two genes from seven gene locus of phenazine were cloned inEscherichia coli DH5α. The contaminated rice samples were collected from infected...

Author(s): Maesomeh Anvari and Mohammad Reza Safari Motlagh

March 2010

Growth, acidification and proteolysis performance of two co-cultures (Lactobacillus plantarum-Bifidobacterium longum and Streptococcus thermophilus-Bifidobacterium longum)

In this study, the fermentation and proteolysis of two co-cultures were investigated. Two fermented cow skim-milk lactoferrin 1 (LF1) and lactoferrin 2 (LF2) were prepared. LF1 was inoculated with Bifidobacterium longum (Bf I) and Streptococcus thermophillus (St I) while LF2 was inoculated with Lactobacillus plantarum (Lb O) and B. longum (Bf I). Incubation was at 42°C for 8 h. The enumeration revealed bacterial...

Author(s): K. Belkaaloul, A. Chekroun, A. Ait-Abdessalam, D. Saidi and Kheroua O.

March 2010

Phylogenetic information reveals the peculiarity of Caryedon serratus (Coleoptera, Chrysomelidae, Bruchinae) feeding on Cassia sieberiana DC (Caesalpinioideae)

Studies over the past 20 years on natural populations indicated that sympatric speciation may be far more common and widespread among plants and animals than previously thought. By using molecular phylogenetics (on a combined data set of two genes), the relationships between Caryedon serratus native forms and forms feeding on groundnut was investigated. The specific objectives were to clarify the taxonomic...

Author(s): M. Sembène, K. Kébé, A. Delobel and J. Y. Rasplus

March 2010

Detection and quantification of probiotic bacteria using optimized DNA extraction, traditional and real-time PCR methods in complex microbial communities

The aim of this study is to optimize molecular detection and quantification methods of probiotic bacteria in complex microbial communities that have long been difficult for traditional culture-based methods. Traditional and real-time PCR were optimized to detect and quantify Lactobacillus spp. andBifidobacterium spp. in complex microbial community. Fish and shrimp sauce were used as a model for complex...

Author(s): Abdulamir A. S., Yoke T. S., Nordin N. and Abu Bakar F.,

March 2010

Isolation and characterization of arsenite oxidizing Pseudomonas lubricans and its potential use in bioremediation of wastewater

A bacterium, Pseudomonas lubricans, isolated from heavy metal laden industrial wastewater, has been shown to tolerate multiple heavy metals suggesting its importance in bioremediation of industrial effluents. P. lubricans tolerated As(III) up to 3 mg ml-1, Cu2+ up to 0.7 mg ml-1, Hg2+ up to 0.4 mg ml-1, Ni2+ up to 0.4 mg ml-1 and Cr6+ up to 0.5 mg ml-1. P....

Author(s): Abdul Rehman, S. Awais Butt and Shahida Hasnain

March 2010

Effect of storage conditions on the shelf life of locally distilled liquor (Akpeteshie)

Locally distilled liquor (Akpeteshie) is a beverage which is widely consumed in Ghana. It is produced from different raw materials. The purpose of this research was to determine the presence and levels of certain contaminants in akpeteshie from the various sources and to determine effects of ambient...

Author(s): Zakpaa, H. D., Mak-Mensah, E. E., and Avio, O. A.

March 2010

Craniometric patterns of three Nigerian ethnic groups

The usefulness of craniometry in facilitating proper identification of skeletal remains and in emphasizing a common origin of studied populations is far reaching. Conducting anthropological studies with the aim of obtaining the characteristics of ethnic groups assists not only in understanding the frequency distribution of human morphologies but also in providing the basis for comparison of races. Craniometric patterns...

Author(s): Odokuma, E. I, Igbigbi, P. S., Akpuaka, F. C. Esigbenu, U. B.

March 2010

Comparision of microscopy and RDTs techniques for laboratory detection of malaria

The development of rapid and specific diagnostic test to identify individuals infected with malaria is important to control severe public health impact of this disease.This study evaluated the ability of rapid malaria diagnostic test (BIOTEC Malaria P.v/P.f rapid device) to detect Plasmodium falciparum and Plasmodium vivax malaria during out break in Chabahar province of Sistan and Balouchestan in...

Author(s): Abdolahi khabisi Samane, Hosein Zadeh Nahid, Shahabi Saaed, Hushang Khazan, Haghighi Ali, Raisii Ahmad, Edrissian Gholam hosein and Abadi alireza

March 2010

Influence of non starch polysaccharide-degrading enzymes on the meat yield and viscosity of jejunal digesta in broilers fed wheat/barley-based diet

This study was conducted to investigate the effect of four commercial multi-enzymes on the performance, meat yield, water intake, litter moisture and jejunal digesta viscosity of chicks fed wheat/barley based diet. A total of 195 1-d-old male broiler chicks (Ross 308) were allocated to 5 treatment groups, with 3 replicates per treatment group and 13 birds per replicate pen and all data were analyzed in a randomized...

Author(s): Hassan Shirzadi, Hossein Moravej, Mahmood Shivazad

March 2010

Follicular and ovulatory responses of the right versus left ovaries to eCG treatment in Shall Iranian ewes

The present study considers the right versus the left ovarian responses to the different doses of eCG treatment in ewe. The estrous cycles of 32 adult Shall Iranian ewes were synchronized by CIDR during breeding period. At the time of CIDR removal, 0 (n = 8), 450 (n = 8), 550 (n = 8) and 650 (n = 8) IU of eCG (Intervet, Booxmer, Netherlands) was administered to ewes. The number of large follicles (> 3 mm, LF) and CL...

Author(s): Hamed Kermani Moakhar, Hamid Kohram, Reza Salehi and Ahmad Zare Shahneh