African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12427

Table of Content: 8 May 2012; 11(37)

May 2012

Potential anticancer activity of the medicinal herb, Rhazya stricta, against human breast cancer

  Breast cancer is a global health problem being the most common and the leading cause of cancer death among women, which mandates the development of novel strategies for its treatment and prevention. Rhazya stricta Decne (Harmal) is an important medicinal plant used in traditional oriental medicine. It has anti-oxidant, anti-carcinogenic, and free radical scavenging properties. Our purpose was...

Author(s): Nabih A. Baeshen, Ayman I. Elkady, Osama A. Abuzinadah and Mohammed H. Mutwakil

May 2012

Cytogenetic studies among Iranian infertile men: The first 20-year long-term report

  Chromosomal aberrations have been postulated to be one of the principal genetic factors in male infertility and occur in about 2 to 3% of unselected patients with proven sub-fertility. This rate is estimated to be 5 to 7% in patients with oligospermia, increasing to 10 to 15% in patients with azoospermia. The aim of this study was to report the frequency of all chromosomal aberrations among Iranian infertile...

Author(s): Cyrus Azimi, Malihea Khaleghian and Farideh Farzanfar

May 2012

Performance of oyster mushroom (Pleurotus ostreatus) in different local agricultural waste materials

  The present study was conducted to evaluate different substrates for establishment of yield performance of oyster mushroom (Pleurotus ostreatus). The substrates were prepared from single or mixture of different agricultural waste materials supplemented with lime and water as additive to each substrate. A total of eight substrates labeled 1 to 8 were prepared. Substrate 4, which is a mixture of palm kernel...

Author(s): Chukwurah, N. F., Eze, S. C., Chiejina, N. V., Onyeonagu, C. C., Ugwuoke, K. I., Ugwu, F. S. O., Nkwonta, C. G., Akobueze, E. U., Aruah, C. B. and Onwuelughasi, C. U.

May 2012

Effectiveness of native Rhizobium on nodulation and growth properties of dry bean (Phaseolus vulgaris L.)

  This study was conducted in order to evaluate the symbiotic effectiveness of nativeRhizobium isolated strains on number of nodule, weight, and morphological properties of dry bean. To realize this purpose, fresh and healthy root nodules were collected from major dry bean-growing districts of Konya, Turkey. These nodules were used to determine the effects of native Rhizobium isolated strains in...

Author(s): Ümmühan Karaca and Refik Uyanöz

May 2012

Temperature impacts on wheat growth and yield in the North China Plain

  To provide base for improving wheat capacity in warmer climate in the North China Plain (NCP), a four-year field experiment was conducted to study temperature effects on length of growth period (LGP), final leaf number (FLN) and yield with six wheat cultivars at 20 test sites (from 32°4´ N to 36°1´ N) of Henan Province in NCP. The results show that...

Author(s): Qiao-Yun LI, Lei LI, Ji-Shan NIU, Jun YIN

May 2012

Effects of nutrient media constituents on growth and development of banana (Musa spp.) shoot tips cultured in vitro

  The objective of this research was to evaluate the effects of nutrient media constituents on growth and development of banana plantlets produced in vitro. Apical buds were excised, disinfected, and cultured in Murashige and Skoog (MS) nutrient medium supplemented with organic compounds at three culture stages. In stage 1, the role of sucrose (15 to 60%), inositol (0 to 1000...

Author(s): Noreldaim Hussein

May 2012

Response of S.C.704 maize hybrid seed production to planting pattern

  In order to determine the best planting pattern for producing the S.C.704 hybrid seed of maize, a field experiment was conducted in 2007 at Safiabad Dezful Research Center via a complete block design with four treatments and replicates each. The treatments were: D1 (one row each of paternal and maternal lines), D2 (two rows of paternal line and one row of maternal line), D3 (two rows each of paternal and...

Author(s): M. Sharafizadeh, M. R. EnayatGholizadeh, N. Aryannia and M. Razaz

May 2012

Role of calcium in tomato (Lycopersicon esculentum Mill.) resistance to Botrytis cinerea

  Tomato (Lycopersicon esculentum Mill.) was cultured in Hoagland’s solution containing 0, 3 or 8 mM Ca2+, to investigate the responses against Botrytis cinereainfection and the effects of Ca2+ on salicylic acid (SA) biosynthesis and signal transduction. The disease index was significantly lower in 8 mM Ca2+ treatment compared to the lower Ca2+ treatment groups. Gene expression...

Author(s): Linlin Li, Tianlai Li, Tao Xu, Mingfang Qi, Zhaoge Yu and Kangkang Zhang

May 2012

Effects of explant type, plant growth regulators and activated charcoal on direct organogenesis of Silybum marianum

  To establish an efficient propagation protocol, plants were regenerated from mature leaf and petiole explants through direct organogenesis without intervening callus phase. The results show that leaf was the best explant for shoot regeneration when cultured on Murashige and Skoog (MS) medium supplemented with 0.2 mgl-1 α-naphthalene acetic acid (NAA), 0.3 mgl-1 zeatin and 0.3...

Author(s): T. Samandari Gikloo, B. Elhami and M. Khosrowchahli

May 2012

Differential responses to salinity stress of two varieties (CoC 671 and Co 86032) of sugarcane (Saccharum Officinarum L.)

  A comparative study was made to assess salt stress responses of sugarcane (Saccharum officinarum L.) var. CoC 671 and Co 86032 using in vitro plantlets by subjecting them to increasing concentrations of NaCl (0, 50, 100, 150, 200 and 250 mM) and checking relative growth rate (RGR), membrane damage rate (MDR), soluble proteins, osmolytes (proline, glycine betaine), ions (Na+ and K+)...

Author(s): Avinash Karpe, Ashok A. Nikam, Krunal P. Chimote, Sachin B. Kalwade , Prashant G. Kawar, Harinath Babu, Rachayya M. Devarumath Penna Suprasanna

May 2012

Influence of different levels of garden compost (garden wastes and cow manure) on growth and stand establishment of tomato and cucumber in greenhouse condition

  Garden compost was mixed with a peat-based growing media (Kimia, Pardis Production and Iran) at rates of 0, 10, 20, 40, 60 and 100% by volume. Garden compost increased electrical conductivity (EC) and pH of soil in the pots, which were slightly higher than the acceptable EC and pH ranges. Cucumber and tomato seeds were planted in complete randomized design with 10 replications. Different substrate...

Author(s): Marzeh Ghanbari Jahromi,Abdolhossein Aboutalebi and Mehdi Hosseini Farahi

May 2012

Reducing power and radical scavenging activities of phenolic extracts from Juglans regia hulls and shells

  Iranian walnut shows genetic diversity and has a large distribution. Different species of these walnuts have many variations in genes resistant to dryness, diseases, pests and other factors. In order to compare the reducing power and radical scavenging activities of Iranian walnut hull and shell phenolic extracts, seven different cultivars (Duclouxiana, Fallax, Orientis, Mayette, Fernor, Mellanaise and Elit)...

Author(s): Amir JALILI, Reza HEYDARI, Asra SADEGHZADE , Shahriar ALIPOUR

May 2012

Effect of different explants on in vitro propagation of gerbera (Gerbera jamesonii)

  This research work involves shoot induction, multiplication of induced shoot and their rooting in Gerbera jamesonii (Gerbera). For shoot induction, apical meristems and vegetative buds were used. Murashige and Skoog (MS) medium containing 6-benzylaminopurine (BAP) alone or in combination with indole-3-acetic acid (IAA) was tested. Best shoot induction response was obtained after 8 days of...

Author(s): Shagufta Naz, Fozia Naz, Amina Tariq, Farah Aslam, Aamir Ali, and Mohammad Athar

May 2012

Comparison of Escherichia coli KO11 and Saccharomyces cerevisiae ATCC 96581 in fermenting Pinus patula hydrolysate pretreated at different steam explosion severity

  Bioconversion of wood residues to biofuels such as ethanol is one of the feasible initiatives towards production of renewable energy. This work compares the effectiveness of Escherichia coli KO11 and Saccharomyces cerevisiae ATCC 96581 in fermenting Pinus patula (pine) hydrolysate pretreated by acid-catalyzed steam explosion. The results show no significant difference in terms of...

Author(s): Nyangi Chacha, Nils Dyrset, Godliving Mtui, Jamidu Katima and Godwill Mrema

May 2012

Dynamic of soil microbial-community: Terminal restriction fragment length polymorphism analysis on natural secondary succession of Pinus tabulaeformis Carr. in the forest region of Loess Plateau, China

  In order to assess the dynamics of microbial-diversity through vegetation successions, sample sites for soil investigation at the 10a, 25a, 40a, 75a natural secondary Pinus tabulaeformis Carr. forests were established in the forest region of Loess Plateau, China. With the succession extension, soil microbial biomass carbon (MBC), moisture content, soil organic carbon (SOC) as well as total...

Author(s): Jin-cheng WANG, Ming-bo JING, Zhong-min DONG, Tian-lin ZHOU and Jian LIANG

May 2012

Biodegradation of used motor oil by single and mixed cultures of cyanobacteria

  This study was carried out to evaluate the potential of single and mixed cultures ofNostoc hatei and Synechocystis aquatilis in the biodegradation of 10% used motor oil. The rates of biodegradation of the oil were studied for a period of 21 days under laboratory conditions. Single cultures of N. hatei performed best in the biodegradation of the oil, showing dramatic reduction in total...

Author(s): Witaya Pimda and Sumontip Bunnag

May 2012

Antidiabetic effect of burdock (Arctium lappa L.) root ethanolic extract on streptozotocin-induced diabetic rats

  Antidiabetic effect of the root ethanolic extract of burdock (Arctium lappa L.), a traditional medicinal and edible plant, was investigated in streptozotocin- induced diabetic rats. The results show that the oral administration of burdock root ethanolic extract (BRE) significantly decreased blood glucose and increased insulin level in diabetic rats compared to the control diabetic group. Meanwhile, the...

Author(s): Jianfeng Cao, Chaopin Li, Pengying Zhang, Xu Cao, Taotao Huang, Yungui Bai and Kaoshan Chen,

May 2012

Effect of iron deficiency on the localization of phosphoenolpyruvate carboxylase in common bean nodules

  Phosphoenolpyruvate carboxylase (PEPC) plays an important role in nodules, when there is an increase in the demand for energy. This enzyme provides carbon skeletons to sustain amino acid synthesis and malate to support energy required to fix nitrogen. Since PEPC is important for nodules, and there is lack of information about the effect of some nutrient deficiency in the expression and localization...

Author(s): Tarek Slatni, Imen Ben Salah, Marta Dell’Orto, Gianpiero Vigani, Saber Kouas Houda Gouia Graziano Zocchi and Chedly Abdelly

May 2012

Engineering taxol biosynthetic pathway for improving taxol yield in taxol-producing endophytic fungus EFY-21 (Ozonium sp.)

  Key enzyme gene transformation is an ordinary and effective method to increase secondary metabolites of plants and microorganisms. In this work, a fungus expression vector (pV2+-TS-pAN7-1) containing taxadiene synthase gene (ts), a rate-limiting enzyme gene for taxol biosynthesis, was constructed and used in the transformation of taxol-producing endophytic fungus EFY-21. Southern blot analysis demonstrated...

Author(s): Yamin Wei, Lu Liu, Xuanwei Zhou, Juan Lin, Xiaofen Sun and Kexuan Tang,

May 2012

Effect of addition of organic and inorganic combinations to soil on growing property of greenhouse cucumber

  This research was carried out using a completely randomized design with seven treatments and seven replications. The treatments were perlite‚ date palm waste, soil, perlite + soil (Pe + s, w/w= 5/95), perlite + soil (w/w = 10/90), date palm waste + soil (w/w = 5/95) and date palm waste + soil (Pa+s, w/w= 10/90). The physiochemical characteristics of substrates were measured before plant cultivation....

Author(s): Ahmad Mohamadi Ghehsareh and Mahmoud Kalbasi

May 2012

Production of ethanol from cellobiose by recombinant β-glucosidase-expressing Pichia pastoris: Submerged shake flask fermentation

  Pichia pastoris strain GS115 was engineered to express a synthetic gene encoding β-glucosidase for its use in ethanol production. β-glucosidase was expressed and secreted either as mature enzyme or N- hexahistidine (His6)-tagged fusion protein, under the control of the strong methanol inducible promoter AOX1. The recombinant β-glucosidase was partially characterized and showed properties...

Author(s): Didier Jean Louis MEKO’O, Yun XING, Li Li SHEN, Guy-Armel BOUNDA, Jie WU, Taiming LI, Rongyue CAO, Jing Jing LIU

May 2012

Development of loop-mediated isothermal amplification (LAMP) and comparison with polymerase chain reaction (PCR) for the detection of goose parvovirus

  Goose parvovirus (GPV) causes high mortality in domestic goslings and Muscovy ducklings which affects geese production worldwide. In this study, loop-mediated isothermal amplification (LAMP) was developed with two pairs of specific primers to detect GPV. The established method proved to be highly sensitive to detect one copy of GPV genome as same as polymerase chain reaction (PCR). No cross-reaction with...

Author(s): Jin Wenjie#, Zhong Lei, Bao Yanqing, Wang Xi, Wang Qianqian, Qin Aijian#, Qian Kun and Shao Hongxia

May 2012

Expression profiling of brca1 gene in familial breast cancer in India

  The incidence of breast cancer in India is on the rise. This was indicated by the recent report from Indian Council of Medical Research wherein it was reported that 1 in 22 women in India was likely to suffer from breast cancer in their life time. About 40 to 50% of hereditary breast cancers and most of hereditary breast and ovarian syndromes are thought to be due to mutation in the breast cancer...

Author(s):   Research Center for DNA Diagnostics, Department of Applied Genetics, Karnatak University, Dharwad - , Karnataka, India. Department of Biotechnology, P. C. Jabin Science College, Vidyanagar – Hubli, Karnataka, India. Center of Excellence in Molecular Hemato-oncology, Department of Applied Genetics, Karnatak University, Dharwad - , Karnataka, India. Karnataka Cancer Therapy and Research Institute, Navanagar - , Hubli, Karnataka, India. Department of Paediatrics, Karnataka Institute of Medical Sciences, Hubli – , Karnataka, India. Department of Anatomy, Karnataka Institute of Medical Sciences, Hubli – , Karnataka, India. Department of Paediatrics, JJM Medical College, Davangere, Karnataka, India. Centre for Cellular and Molecular Biology, Hydrabad, Andhra Pradesh, India.

May 2012

Types and distribution of mucous cells of the abalone Haliotis diversicolor

  The types and distribution of mucous cells of Haliotis diversicolor were observed and analyzed using the alcian blue and periodic acid schiffs (AB-PAS) reaction and histological procedures. According to the color of the mucous cells, they were divided into four types: Type I, pure red; type II, pure blue; type III, purple reddish; type IV, blue purple. Most of the mucous cells in the mantle were...

Author(s): Guilan Di, Jianbin Ni, Zhaoxia Zhang, Weiwei You, Bo Wang and Caihuan Ke

May 2012

Use of petal explants for successful transformation of Dendranthema × grandiflorum Kitamura 'Orlando' mediated by Agrobacterium tumefaciens

  An Agrobacterium-mediated genetic transformation system for chrysanthemum ‘Orlando’ using petal explants was developed. After decontamination, petals were divided into two parts; terminal (position 1) and middle (position 2) parts, and were pre-cultured for two days on Murashige and Skoog (MS) medium containing 1.0 mg·L-1 indole acetic acid (IAA), 1.0...

Author(s): Ju Yeon Song, Iyyakkannu Sivanesan, and Byoung Ryong Jeong,

May 2012

Morphological characteristics of ladybird beetles collected from District Dir Lower, Pakistan

  A survey was conducted in District Dir Lower, over a period of two years. Specimens of coccinellid beetles were collected from four major localities viz; Maidan, Jandool, Timergara and Adenzai. Identification of these beetles showed that 14 different species in 12 genera belonging to sub-families Coccinellinae, Chilocorinae, Scymninae and Epilachninae are present in the selected area. In the sub-family...

Author(s): Rahat Ullah, Faizul Haq, Habib Ahmad, Mian Inayatullah, Kausar Saeed and Shahroz Khan

May 2012

Effect of different oestrus synchronizations protocols on the reproductive efficiency of Dammar ewes in Yemen during winter

  This study compared the effect of oestrus synchronization using controlled-internal-drug-release-device (CIDR) and equine chorionic gonadotropin (eCG) on reproductive efficiency of Dammar ewes in winter. Data of forty-one ewes were divided into two groups; for long CIDR treatment (12 days, LT, n=25) and for short CIDR treatment (6 days, ST, n=16). The LT group was further divided into 3 subgroups to receive...

Author(s):   S. O. Nasser, , H. Wahid, A. S. Aziz, A.B. Zuki, M. K. Azam, A. G. Jabbar and M. A. Mahfoz