African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12180

Table of Content: 9 August 2010; 9(32)

August 2010

Recent progress in the differentiation of bone marrow derived mesenchymal stem cells (BMMSCs) to cardiomyocyte- like cells and their clinical application

Bone marrow mesenchymal stem cells (BMMSCs) are one of the cells found in bone marrow stromal. A large number of studies have shown that BMMSCs cannot only differentiate into hematopoietic stromal cells, but can migrate and position themselves in multiple non-hematopoietic organizations and differentiate into the corresponding tissue cells; this characteristic demonstrates their multilineage differentiation potential....

Author(s): Xue-bo Yan, An-lin Lv, Yu-jie Xing, Li Wang and Wei Zhao

August 2010

Inter Simple Sequence Repeat (ISSR) analysis of wild and cultivated rice species from Ethiopia

The genetic diversity of three wild rice populations of Ethiopia along with three cultivated rice populations were studied using Inter simple sequence repeats (ISSRs) as a molecular marker. A total of 93 clear and reproducible bands were generated using four dinucleotides and two tetra nucleotides primers. Both UPGMA and neighbor joining trees were constructed for each individual and population using...

Author(s): Gezahegn Girma, Kassahun Tesfaye, and Endashaw Bekele

August 2010

Functional characterization of a gluthathione S-transferase gene from Limonium bicolor in response to several abiotic stresses

In the present study, we characterized a gluthathione S-transferase gene (LbGST1) in response to different abiotic stresses. Real time polymerase chain reaction (RT-PCR) indicated that the LbGST1 can be differentially regulated by stress of NaCl, NaHCO3 and low temperature, suggesting a role in plant stress response. To further investigate abiotic stress tolerance of LbGST1, the transgenic yeast...

Author(s): Guiping Diao, Yucheng Wang and Chuanping Yang

August 2010

Expression of human interferon gamma in Brassica napus seeds

Expressions of heterologous proteins in suitable plant tissues and targeting it into subcellular compartments using specific signals have been studied. Seed-based platforms are among those that allow recombinant proteins to stably accumulate at a relatively high concentration in a compact biomass. In this study, we used seed specific promoter (Napin) and C-terminal KDEL sequence to express human therapeutic protein,...

Author(s): Khadijeh Bagheri, Mokhtar Jalali Javaran, Fereidoun Mahboudi , Ahmad Moeini and Alireza Zebarjadi

August 2010

Assessment of genetic diversity in French bean (Phaseolus vulgaris L) based on RAPD marker

RAPD molecular markers were used to evaluate the genetic diversity in the fourteen varieties of French bean (Phaseolus vulgaris) of three eco-geographical regions of Bangladesh. Out of the 20 primers only, 6 yielded polymorphic banding patterns. In total, 40 different DNA bands were reproducibly obtained, out of which 28 (70%) were polymorphic. The polymorphisms were scored and used in band-sharing...

Author(s): M. S. Biswas, J. Hassan and M. M. Hossain

August 2010

Optimization of fed-batch fermentation for a staphylokinase-hirudin fusion protein in Escherichia coli BL21

In this study, the fed-batch fermentation technique was applied to improve the yield of STH, a chimeric protein composed of staphylokinase (SAK) and hirudin (HV) with thrombin recognition peptide as a linker peptide, produced by Escherichia coli BL21. Three kinds of feeding mediums, GM (glucose + magnesium sulfate), GMY (GM + yeast extract) and GMYT (GMY + tryptone), were investigated with R-medium as an...

Author(s): Guangman Zhang, Genshen Zhong, Xiudong Wang, Lisheng Wang, Yide Qin and Aiping Yu

August 2010

Molecular characterization of Cymbidium kanran cultivars based on extended random amplified polymorphic DNA (ERAPD) markers

Fifty-four Cymbidium kanran cultivars from China, Japan and Korea were examined and analyzed by using the successive screening of 3′-end extended random primer amplified polymorphic DNA (ERAPD) markers to determine their molecular diversity and relationships. In ERAPD analyses, the strand-specific DNA sequence of direct oligonucleotide extension sequencing primers was independently read from each of...

Author(s): Li Jian, , Yi-Zhong Zhang, Dan-Feng Yu and Li-Quan Zhu

August 2010

Opportunities in utilization of agricultural residues in bio-composite production: Corn stalk (Zea mays indurata Sturt) and oak wood (Quercus Robur L.) fiber in medium density fiberboard

In this paper, corn stalk as an agricultural residue was mixed with oak wood fiber to produce medium density fiberboards (MDF). Urea formaldehyde resin was used as binder. Hygroscopic and mechanical properties were evaluated according to the commercial standards in MDF production. Partial substitution of wood fiber with corn stalks fiber negatively affected all board properties. However, the mechanical properties...

Author(s): Mehmet Akgül, Cengiz Güler and Birol Üner

August 2010

Effects of drying methods of lamellas used in multilayer parquet manufacturing on surface roughness and bonding strength

The objective of this study is to determine surface roughness and bonding (tensile shear) strength of lamellas (top layer of multilayer parquet) which were cut away from green lumber and dried by different types of drying methods. Also, finding out the most convenient manufacturing method as surface roughness, bonding strength properties was aimed by comparing results with surface roughness and bonding strength of...

Author(s): Nur Müge Güngör, Süleyman Korkut and Ramazan Kantay

August 2010

The effects of different nitrogen doses on yield, quality and leaf nitrogen content of some early grape cultivars (V. vinifera L.) grown in greenhouse

Nitrogen deficiency is a worldwide problem, causing restrictions in productivity of many horticultural produces. Particularly, the issue is compounded when the greenhouse production is employed. Therefore, reliable knowledge on proper application of nitrogen ensures not only satisfactory yield but also balanced vegetative and reproductive growth in plants. This study was thus conducted to investigate the effects of...

Author(s): Hatice Bilir Ekbic, Gultekin Ozdemir, Ali Sabir and Semih Tangolar

August 2010

Effect of tannery effluents on seed germination and growth of two sunflower cultivars

The wastewater of a tannery in Multan, Pakistan, was alkaline with high biochemical oxygen demand (BOD) and chemical oxygen demand (COD) values along with much higher concentrations of total settle able salts and suspended solids, sodium adsorption ratio and high amount of sodium having the water quality class C3S1. Effluent was examined for its chemical constituents and the effect of its various dilutions was examined...

Author(s): Faiz Hussain, Saeed A. Malik, Mohammad Athar, Nahidah Bashir, Uzma Younis, Mahmood-ul-Hassan and Seema Mahmood

August 2010

In vitro bulblet regeneration from immature embryos of Muscari azureum

A high frequency bulblet regeneration was achieved for endemic and endangered ornamental plant Muscari azureum using immature embryos. Immature embryos ofM. azureum were cultured on callus induction medium consisting of N6 mineral salts and vitamins, 400 mg/L casein + 40 g/L sucrose + 2 g/l L-proline, 2 mg/L 2,4-D and 2 g/L gelrite. Then, embryogenic callus clusters were transferred to bulblet...

Author(s): Serkan Uranbey

August 2010

Effects of boron and gibberellic acid on in vitro pollen germination of pistachio (Pistacia vera L.)

This study was conducted on male pistachio cultivars which consisted of Uygur, Atli, Kaska, Sengel and Kavak to study the influence of boron and gibberellin on pollen germination in vitro. Pollen was sown in germination media that included 20% sucrose, 10, 25, 50, 75 and 100 ppm boric acid (H3BO3) and gibberellic acid (GA3), separately. It was found that pollen germination for all cultivars were greatly...

Author(s): Izzet Acar, Bekir Erol Ak and Kamil Sarpkaya

August 2010

Path analyses of yield and some agronomic and quality traits of bread wheat (Triticum aestivum L.) under different environments

This research was conducted to determine characters effecting grain yield in fifty bread wheat (Triticum aestivum L.) cultivars and advanced lines by using simple correlation coefficient and path analysis under 2 locations (high rainfall and low rainfall; 745 and 506 mm, respectively). A total of 50 genotypes, 25 for each independent experiment, were tested for grain yield, test weight, 1000-kernel weight, Zeleny...

Author(s): Nevzat Aydin, Cemal Şermet,  Zeki Mut, H. Orhan Bayramoğlu and Hasan Özcan

August 2010

Statistical optimization of cultural conditions for chitinase production from fish scales waste by Aspergillus terreus

Aspergillus terreus, a local isolate from fishery polluted soil, was used successfully for the biodegradation of parrot fish-scales waste in favor of the production of highly active chitinase enzyme. Chitinase production was noticeably influenced by the culture medium and the highest enzyme production was attained through the acceleration growth phase (96 h). Pronounced decrease in chitinase production was...

Author(s): Ghanem K. M, Al-Garni S. M and Al-Makishah N. H

August 2010

Application of sand and geotextile envelope in subsurface drip irrigation

Subsurface drip irrigation is a technology used for better management of irrigation water. This technique is more desirable in arid and semi-arid region, because of decreasing soil surface evaporation and more efficiency of irrigation system in comparison with the other irrigation systems. In addition, this system is a safer way for treated wastewater (TWW) reuse in irrigation lands. But roots and clay particles...

Author(s): P. Najafi and S. H. Tabatabaei

August 2010

Evaluation of a pilot scale high pressure plasma ozonizer for use in wastewater treatment

The plasma technique which is used for wastewater treatment is one of the most effective processes for ozone production. In this study, a laboratory scale plasma technique ozonizer designed for treating wastewater was tested under various operation conditions which included voltage (E), current frequency (f), electrical current (I), gas pressure in the system (P), distance of electric dipoles (d), type of electric pole...

Author(s): Mongkol Jongsuphaphong, Suntud Sirianuntapiboon and Bundit Limmeechokchai

August 2010

Production of extracellular amylase from agricultural residues by a newly isolated Aspergillus species in solid state fermentation

The production of extracellular amylases by solid state fermentation (SSF) was investigated employing our laboratory isolate Aspergillus sp.MK07. Various agricultural residual substrates like wheat bran, rice bran and green gram husk were studied for enzyme production. Highest enzyme production was obtained with wheat bran as a substrate. Effects of process variables, namely: incubation period, temperature,...

Author(s): Murali Krishna Chimata, Sasidhar P and Suresh Challa

August 2010

Strain improvement of Gluconacetobacter xylinus NCIM 2526 for bacterial cellulose production

The present investigation demonstrates the effectiveness of ultraviolet (UV) radiation and ethyl methanesulfonate (EMS) in strain improvement for enhanced cellulose production by Gluconacetobacter xylinus NCIM 2526. The mutants were compared with wild type for cellulose production. UV mutants GHUV3, GHUV4, and GHUV5 of G. xylinus showed higher cellulose yield than the wild strain. The mutant...

Author(s): Basavaraj S. Hungund and S. G. Gupta

August 2010

Evaluation of total phenolics, anthocyanins and antioxidant capacity in purple tomatillo (Physalis ixocarpa) genotypes

Purple tomatillo genotypes were evaluated for their total anthocyanin, phenolic and antioxidant capacity. The result showed that ICTS-UDG-9-224 and ICTS-UDG-9-32 had the highest amount of total phenolic compounds 10.08 and 9.6 mg GAE/g fresh weight in genotypes, respectively, followed by ICTS-UDG-1-1 and ICTS-UDG-2-2 (5.5 and 5.3 mg GAE/g fresh weight), respectively. The highest content of anthocyanins was found in...

Author(s): Daniel González-Mendoza Onecimo Grimaldo-Juárez, Roberto Soto-Ortiz, Fernando Escoboza-Garcia and José Francisco Santiguillo Hernández

August 2010

Comparative studies on the in vitro antioxidant properties of methanolic and hydro-ethanolic leafy extracts from eight edible leafy vegetables of Ghana

Epidemiological studies indicate that consumption of fruits and vegetables has the ability to inhibit the damaging activities of free radicals in the human body. Eight edible leafy vegetables of Ghana namely: Xanthosoma sagittifolium, Hibiscus Sabdariffa, Solanum macrocarpon, Talinum triangulare, Corchorus olitorius,Laportea aestuans, Ipomoea batatas, and Amaranthus...

Author(s): Joseph Francis Morrison and Sylvester Kwadwo Twumasi

August 2010

The evaluation of the bone graft survival status in titanium cervical cages by radionuclide bone CT scan

To find a better way to evaluate the bone graft survival status in cervical cages, forty-one patients suffering from one-level cervical spondylosis were enrolled in this study. All underwent anterior cervical decompression and fusion with titanium cage and plate. When followed up, another 21 patients were confirmed as one-level cervical spondylosis without operation and were enrolled as control group. "Bolus"...

Author(s): Suo-chao Fu, Zi-xiang Wu, Shi-yong Wan, Ming-xuan Gao, Da Liu and Wei Lei

August 2010

Cadmium, lead, arsenic and selenium levels in patients with type 2 diabetes mellitus

There is accumulating evidence that the metabolism of several trace metals are altered in diabetes mellitus and these micronutrients might have specific roles in the pathogenesis and progression of the disease. The aim of this study was to investigate the level of toxic elements: lead (Pb), cadmium (Cd) and arsenic (As) levels in whole blood and selenium (Se) (an antioxidant element) in serum of patients with type 2...

Author(s): O. Akinloye, K. Ogunleye and O. O. Oguntibeju

August 2010

Evaluation of trace elements in pregnant women with pre-eclampsia

Trace elements such as zinc, copper, and selenium display antioxidant activity and act as peroxynitrite scavenger while others such as manganese and magnesium are essential micronutrients. The disturbance in the metabolism of these elements may be a contributing factor in the development of certain diseases such as pre-eclampsia. The aim of this study is to determine the status of these elements in pre-eclamptic...

Author(s): O. Akinloye, O. J. Oyewale and O. O. Oguntibeju

August 2010

Development of efficient recirculation system for Tilapia (Oreochromis niloticus) culture using low cost materials

In the current experiment, a recirculation system was built using low cost materials that are available locally and its performance was tested. The performance evaluation procedure was carried out in an aquaculture system in greenhouse withsex-reversed male Nile tilapia (Oreochromis niloticus) production in Querétaro State, Mexico. The recirculation system had four sections (sediment collector, gravel and...

Author(s): M. Genaro Soto-Zarazúa, Gilberto Herrera-Ruiz, Enrique Rico-García, Manuel Toledano-Ayala, Rocío Peniche-Vera, Rosalía Ocampo-Velázquez and Ramón G. Guevara-González

August 2010

Nitric oxide radical scavenging potential of some Elburz medicinal plants

Some plants scavenge nitric oxide (NO) with high affinity. For this purpose, forty extracts from 26 medicinal plants, growing extensively in Elburz mountains, were evaluated for their NO scavenging activity. Total phenolic and flavonoid contents of these extracts were also measured by Folin Ciocalteu and AlCl3 colorimetric assays, respectively. Sambucus ebulus fruit aqueous extract...

Author(s): M. A. Ebrahimzadeh, S. F. Nabavi, S. M. Nabavi, and F. Pourmorad

August 2010

Estimation of genetic parameters for body weight at different ages in Mehraban sheep

The objective of the present study is to estimate genetic parameters of birth weight (BW, n = 3005), weaning weight (WW, n = 2800), 6 months weight (6 MW, n = 2600), 9 months weight (9 MW, n = 1990) and yearling weight (YW, n = 1450) of Mehraban sheep, collected during 1995 - 2007 at Mehraban sheep Breeding Station in Hamedan  province, Iran. (Co)variance components and genetic parameters were estimated with...

Author(s): Vali Aghaali Gamasaee, Seyed Hasan Hafezian, Ahmad Ahmadi, Hasan Baneh, Ayyoob Farhadi and Alborz Mohamadi

August 2010

The role of intraperitoneally administered vitamin C during Trypanosoma congolense infection of rabbits

The effects of daily intraperitoneally administered doses of 100 mg/kg bd. wt. vitamin C on levels of some endogenous antioxidants as well as hepatic and renal function were investigated in a group of rabbits infected with a strain ofTrypanosoma congolense (strain number: BS2/TC/SP28/P4). Values of parameters estimated in this group during and after 5 weeks of infection were compared with those from a group of...

Author(s): Ismaila A. Umar, Ibrahim Toma, Caroline A. Akombum, Chinelo J. Nnadi , Mohammed A. Mahdi, Abubakar Gidado, Ikechukwu O. Igbokwe and Lawan B. Buratai

August 2010

Interaction of avian infectious bronchitis virus S1 protein with heat shock protein 47

Infectious bronchitis (IB) caused by a coronavirus is an important disease in chickens; it mainly affects respiratory and kidney systems. The IBV-S1 protein is an important structural and functional protein. To identify the interaction between the IBV-S1 proteins and to elucidate the possible involvement of S1 protein in IBV pathogenesis, a chicken kidney cDNA library was screened using a yeast two-hybrid system assay....

Author(s): Pengju Zhang, Hongning Wang, Zhiguang Zeng, Lang Feng, Yinglin Liu and Haipeng Cao

August 2010

Ovarian follicular dynamics during the interovulatory interval in Najdi goats

The pattern of growth and regression of ovarian follicles was characterized during the estrus cycle in 5 Najdi goats by daily ultrasonographic examination of the ovaries. Follicles ≥2 mm was measured and their relative locations within the ovary were determined to follow the sequential development of each individual follicle. Results indicated the presence of either four (n = 2 estrus cycles) or five (n = 3...

Author(s): Godratollah Mohammadi, Hamid Kohram, Saad Gooraninejad, Alireza Yousefi and Arash Motaghedi