African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12072

Table of Content: 9 October 2013; 12(41)

October 2013

Bacterial species identification getting easier

  The traditional methods of bacterial identification are based on observation of either the morphology of single cells or colony characteristics. However, the adoption of newer and automated methods offers advantage in terms of rapid and reliable identification of bacterial species. The review provides a comprehensive appreciation of new and improved technologies such fatty acid profiling, sequence analysis of...

Author(s): Phumudzo Tshikhudo, Ronald Nnzeru, Khayalethu Ntushelo and Fhatuwani Mudau

October 2013

Genetic stability of mulberry germplasm after cryopreservation by two-step freezing technique

  Genetic stability of Morus alba, Morus indica, Morus laevigata (indigenous collection) and Morus species (exotic collection) have been studied in in vitro regenerated plants of mulberry (fresh, before and after cryopreservation) using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. This study examined the...

Author(s): Ravish Choudhary, Rekha Chaudhury, S. K. Malik, Susheel Kumar and Digvender Pal

October 2013

Expression of an engineered tandem-repeat starch-binding domain in sweet potato plants

  In this study, the transgenic sweet potato Xu55-2 modified with an engineered tandem repeat of a family 20 starch binding domain (SBD2) was analyzed by Western dot blot to investigate whether SBD2 proteins are capable of granule-targeting during starch biosynthesis. Furthermore, the impact of SBD2 accumulation in granules on the physicochemical properties of the transgenic starches was also investigated. Our...

Author(s): Yunfeng Zhang, Qin Ji, Yujun Xing, Qiang Li, Xin Wang,  Chun Xu and  Cuixiang  Huang

October 2013

Cytogenotoxic effects of cypermethrin, deltamethrin, lambdacyhalothrin and endosulfan pesticides on Allium cepa root cells

  Increased pesticides application in agriculture and public health has contributed to the pollution of the environment. This study evaluates the cytogenotoxic effects of emulsifiable concentrate of cypermethrin, deltamethrin, lambdacyhalothrin and endosulfan on Allium cepa root cells. Five concentrations (1.0, 5.0, 10.0, 20.0 and 40.0 ppm) of each pesticide were used for microscopic (48 h) and...

Author(s): Yekeen, Taofeek A. and Adeboye, Messach K.

October 2013

Effects of sulphur preservative on phytochemical and antioxidant capacity of peels of mango cultivars (Mangifera indica L.) produced in South Africa

  Fruits have abundant phytochemicals that contribute as bioactive molecules with ability to lower incidence of diseases. Mangoes are rich in polyphenols and antioxidants. In this study, peels of six selected mango cultivars (Tommy Atkins, Peach, Saber, Sunshine, Keitt and Vhavenda) were treated with sulphur dioxide solutions (0, 10, 20, 50, 100, 150, 200, 250 and 300 ppm) as preservative...

Author(s): Mudau Fhatuwani Nixwell, Mabusela Johanna and Wonder Ngezimana

October 2013

Physicochemical changes of raffia sap (Raphia mambillensis) contents during spontaneous fermentation

  The chemical content of a substance can influence its shelf life. Fermentation causes changes in freshly tapped sap and therefore makes its large scale use difficult.  In order to study the effect of time on fermentation on the raffia sap property, its physico-chemical and microbiological characteristics were determined during five days. The results recorded for the harvest reveal...

Author(s): Tiepma,  N. E. F.,  Zambou, N. F., Agbor, E. E., and Tchouanguep, M.  F.

October 2013

Tuberculosis treatment raises total cholesterol level and restores high density lipoprotein cholesterol (HDL-C) in patients with pulmonary tuberculosis

  The aim of this study was to determine whether tuberculosis (TB) treatment normalizes the lipid profile strongly affected by pulmonary TB. Serum levels of total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and triglycerides (TG) were determined in 83 patients with pulmonary TB before and after treatment, and compared to results obtained from 100...

Author(s): AKPOVI Dewanou Casimir, GBAGUIDI Lucette H. Senami, ANAGO Eugénie, AFFOLABI Dissou, DOUGNON Tamègnon Victorien, FAIHUN Franck and ANAGONOU Sévérin,

October 2013

Mechanism of ciprofloxacin resistance in Shigella dysenteriae

  Bacterial resistance to quinolones has emerged rapidly and such resistance has traditionally been attributed to the chromosomally mediated mechanisms that alter the quinolone targets and/or over-produce multidrug resistance efflux pumps. In thepresent investigation possible mechanism of ciprofloxacin (Cp) resistance in Shigella dysenteriae was studied. While the growth of sensitive S....

Author(s): Nilima Lankeshwar and Uttamkumar S. Bagde

October 2013

Morphology and anatomical structure of the larval salt gland of Artemia tunisiana under different salinities

  Brine shrimps of the genus Artemia is characterized by its high adaptability to adverse environmental conditions. To elucidate the effect of salinity on the neck organ (salt gland) of Artemia tunisiana nauplii, the morphology and fine structure of the ion transporting epithelium were examined following culturing under different salinities (25, 40, 70, 140 and 180 g/L). The expression...

Author(s): Magdy E. Mahfouz, Mona M. El-Gamal and Suzan M. Shraby

October 2013

Study of seasonal sexual activity variations in Algerian rams: Sexual behaviour, testosterone concentration control and environmental factors

  This study focuses on the determination of seasonal effect on two main andrological sexual activity parameters within young and adults rams of Rembi breed from Algeria for a period of one year. The experiment involved a weekly evaluation of males’ sexual behaviour and a monthly measurement of serum testosterone concentration in order to know...

Author(s): Benia, A. R., Taibi, K., Ait-Amrane, A., Belhamiti, T., Hammoudi, S. M. and Kaidi, R.