July 2003

Serological versus molecular diagnosis

Author(s): N. J. Tonukari

July 2003

Production of monoclonal and polyclonal antibodies against a Nigerian isolate of banana streak virus

  Banana streak virus is serologically and genomically heterogenous worldwide and there has been the need to produce antibodies that can detect all known serotypes of this virus. Antibody production requires purified virus, since BSV titre is low in Musa tissues, there was the need for an efficient method of purifying the virus. We are reporting the first production of two monoclonal antibodies, BSV...

Author(s): Agindotan B.O. , Thottappilly G.,  Uwaifo A and Winter S

July 2003

Influence of cultural conditions on the production of bacteriocin by Lactobacillus brevis OG1

  Bacteriocin produced by Lactobacillus brevis OG1 has large spectrum of inhibition against pathogenic, food spoilage microorganisms and various Lactic acid bacteria employed as test strains. The bacteriocin inhibited E coli NCTC 10418 andEnterococcus faecalis, but did not inhibit Candida albicans ATCC 10231 andKlebsiella sp. UCH 15.  The antibacterial activity...

Author(s): S.T.  Ogunbanwo, A.I. Sanni, and A. A. Onilude

July 2003

Analysis of AVR4 promoter by sequential response-element deletion

  Several reports have associated the variability in physico-chemical properties of avidin protein to dynamism inherent in the consensus regulatory networks within the promoter region of avidin genes. An Avr4 promoter region ligated to chloramphenicol acetyltransferase plasmid vector (pBLCAT2) to produce recombinant plasmid Avr4pBLCAT2 was sequentially deleted to produce five distinct mutants:...

Author(s): Olukosi, YA and Iwalokun, BA

July 2003

Efficacy of IgG, Fab, and F(ab')2 fragments of horse antivenom in the treatment of local symptoms after Cerastes cerastes (Egyptian snake) bite

  The ability of horse antivenoms, consisting of immunoglobulin G (IgG) and its fragmentsF(ab')2 and Fab were comparatively studied in mice to neutralize several effects ofCerastes cerastes venom.  The three antivenoms were produced from the same batch of hyperimmune horse plasma. Neutralization was only partial when antivenins were administered intravenously at various time...

Author(s):   Salwa S. Abdel Latif, Soheir Wanas, George Abdel Malak and Madiha H. Helmy

July 2003

Genetics similarity among four breeds of sheep in Egypt detected by random amplified polymorphic DNA markers

  A genetic analysis using RAPD markers was performed for studying variation in four breeds of sheep (Baladi, Barki, Rahmani and Saffolk). Nineteen random primers were used to amplify DNA fragments in these breeds. RAPD patterns with a level of polymorphism were detected between breeds. Results showed closer proximity of Barki to Rahmani and Baladi (95.7 and 91.3%),...

Author(s): Bahy Ahmed Ali

July 2003

Nodulation and nitrogen fixation of field grown common bean (Phaseolus vulgaris) as influenced by fungicide seed treatment

  A field experiment was conducted at Bel Air station, in Dakar using 15N isotope dilution technique and the non nodulating soybean (Glycine max) variety m129 as reference plant to test the compatibility of Dichlorofenthion-thiram (DCT) fungicide to the inoculation of common bean (Phaseolus vulgaris) Paulista variety with both Rhizobium etli ISRA 353 and R. tropici strain ISRA...

Author(s):   Ndeye Fatou Diaw GUENE, Adama DIOUF and Mamadou GUEYE

July 2003

Inheritance of resistance to head bug (Eurystylus oldi) in grain sorghum (Sorghum bicolor)

  The inheritance of resistance to head bug (Eurystylus oldi) was studied in ten populations of sorghum derived from crossing three susceptible sorghum elite varieties (ICSV 111, ICSV 112 and ICSV 400), and two resistant sorghum varieties (Malisor 84-7 and KSV 4). Parental lines, F1 and F2 populations were sown on a Randomized Complete Block Design in two replications. Artificial infestation...

Author(s): S. E. Aladele and I. E. Ezeaku

July 2003

Comparison of multi-locus enzyme and protein gel electrophoresis in the discrimination of five Fusarium species isolated from Egyptian cottons

  Electrophoretic studies of multilocus-enzymes (MLEE) and whole-cell protein (SDS-PAGE) were carried out in order to evaluate the parity between different methods for the characterization of five Fusarium species recovered from cotton-growing areas in Egypt by numerical taxonomy methods. The obtained data revealed that SDS-PAGE and esterase isozymes are more efficient in grouping isolates in their...

Author(s):   Ibrahim N. Aly,  Mohmed A. Abdel-Sattar, Kamel A. Abd-Elsalam, Mohmed S. Khalil and Joseph A. Verreet