March 2003
Molecular phylogeny of Fusarium species by AFLP fingerprint
The high-resolution genotyping method of amplified fragment length polymorphism (AFLP) analysis was used to study the genetic relationships within and between natural populations of five Fusarium spp. AFLP templates were prepared by the digestion ofFusarium DNA with EcoRI and MseI restriction endonucleases and subsequent ligation of corresponding...
March 2003
Biodeterioration of the African star apple (Chrysophylum albidum) in storage and the effect on its food value
The biodeterioration of the African star apple fruits in storage was investigated at Ibadan, southwestern Nigeria. Eight fungal isolates were found associated with the deteriorating fruits. The fungi are Botryodiplodia theobromae, Rhizopus stolonifer,Aspergillus niger, A. tamarii, A. flavus, Fusarium spp, Penicilium spp andTrichoderma spp. All the fungal...
March 2003
Production of alkaline protease by Teredinobacter turnirae cells immobilized in Ca-alginate beads
The conditions for immobilizing the new alkaline protease-producing bacteria strainTeredinobacter turnirae by entrapment in calcium alginate gel were investigated. The influence of alginate concentration (20, 25 and 30 g/l) and initial cell loading (ICL) on enzyme production were studied. The production of alkaline protease improved significantly with increasing alginate concentration and reached a...
March 2003
A capillary electrophoretic method for isolation and characterization of grape xylem proteins
European (Vitis vinifera) and American (Vitis labrusca) grape species succumb to a bacterial disease known as Pierce’s Disease (PD). In contrast, muscadine grape genotypes (Vitis rotundifolia) are tolerant/resistant to PD. This is due to the unique biochemical composition of muscadine xylem. However, because of low protein concentration, conventional methods such as low-pressure chromatography and...
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