African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 383

Full Length Research Paper

Statistical optimization of L–glutaminase production by Trichoderma species under solid state fermentation using African locust beans as substrate

Musa Olatunji Olarewaju
  • Musa Olatunji Olarewaju
  • Department of Biochemistry, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Nigeria.
  • Google Scholar
Humphrey Chukwuemeka Nzelibe
  • Humphrey Chukwuemeka Nzelibe
  • Department of Biochemistry, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Nigeria.
  • Google Scholar


  •  Received: 24 January 2019
  •  Accepted: 20 February 2019
  •  Published: 31 July 2019

Abstract

Six strains of Trichoderma species isolated from soil and air samples were screened for extracellular L-glutaminase production. The highest enzyme producing isolate, Trichoderma longibrachiatum was subjected to screening and nutrients optimization experiments under solid state fermentation using a constant weight of African locust beans as substrate. A specific activity of 54.96 U/mg was achieved at 1% (w/v) glutamine, 0.5% (w/v) glucose, 1% (w/v) NaCl, pH 3.0 and 1.4 ml inoculums size respectively after 3 days of incubation at 27°C as the optimum condition for L–glutaminase production. Asides glutamine, other nitrogen sources; malt extract and ammonium sulphate had a negative influence on enzyme production likewise the supplemented sources of metal ions; zinc and iron (II) ions.

 

Key words: Trichoderma, L-glutaminase, fermentation, African locust beans.