African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 407

Full Length Research Paper

The effect of aqueous extract of zest of citrus sinensis (AEZCs) on cadmium chloride induced liver toxicity in wistar rats

Augustine Ndubuisi
  • Augustine Ndubuisi
  • Department of Medical Biochemistry, College of Medicine University of Nigeria, Enugu Campus, Enugu State, Nigeria.
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Ani Celestine
  • Ani Celestine
  • Department of Physiology, College of Medicine, Enugu State University of Science and Technology, Parklane Enugu, Nigeria.
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Eze Wenceslaus
  • Eze Wenceslaus
  • Department of Medical Biochemistry, College of Medicine, Enugu State University of Science and Technology, Parklane Enugu, Nigeria.
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Ugwudike Patrick
  • Ugwudike Patrick
  • Department of Medical Biochemistry, College of Medicine, Enugu State University of Science and Technology, Parklane Enugu, Nigeria.
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Anyaeji Pamela
  • Anyaeji Pamela
  • Department of Human Physiology, College of Medicine University of Nigeria, Enugu Campus, Enugu State, Nigeria.
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Ude Victor Chibueze
  • Ude Victor Chibueze
  • Department of Medical Biochemistry, College of Medicine, Enugu State University of Science and Technology, Parklane Enugu, Nigeria.
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Agu Francis Uchenna
  • Agu Francis Uchenna
  • Department of Human Physiology, College of Medicine University of Nigeria, Enugu Campus, Enugu State, Nigeria.
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Nworgu Choice
  • Nworgu Choice
  • Department of Human Physiology, College of Medicine University of Nigeria, Enugu Campus, Enugu State, Nigeria.
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Ikwuka David
  • Ikwuka David
  • Department of Human Physiology, College of Medicine University of Nigeria, Enugu Campus, Enugu State, Nigeria.
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Ugwuishi Emeka
  • Ugwuishi Emeka
  • Department of Physiology, College of Medicine, Enugu State University of Science and Technology, Parklane Enugu, Nigeria.
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Nwachukwu Daniel
  • Nwachukwu Daniel
  • Department of Medical Biochemistry, College of Medicine University of Nigeria, Enugu Campus, Enugu State, Nigeria.
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  •  Received: 21 August 2019
  •  Published: 31 January 2020

Abstract

The potency of the peels of Citrus sinensis against cadmium induced liver damage has not been explored in our environment. 48 wistar rats were used for this study. The animals were randomly divided into eight (8) groups of five (5) rats each. Group A was the positive control and received 5 mg/kg body weight (bw) of cadmium chloride (CdCl2) intraperitoneally as a single dose. Groups B and C received the aqueous extract of zest of citrus sinensis (AEZCS) at a low doses of 10   and 40 mg/kg bw respectively. Groups D and E received cadmium chloride, followed by low and high doses of AEZCS respectively. Groups F and G received low and high doses of AEZCS followed by CdCl2 while group H served as the normal control. Liver enzymes (AST, ALT and ALP) and serum total proteins were analyzed. The results showed significant (P<0.05) differences in the mean values of LV/BW, ALT, AST, total proteins, serum dismutase (SOD) and malondialdehyde (MDA) when the positive control group was compared with the normal control group (P<0.05). Histological sections of the negative control groups were significantly different from the positive control group but not from the groups treated with AEZCS at the high doses. Thus, AEZCS had ameliorative and protective health benefits at the high dose of 40mg/kg body weight.

 

Key words: Citrus sinensis, oxidative stress, cadmium chloride, hepatotoxicity.