African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 391

Full Length Research Paper

Evaluationof in vivo toxicity of rice husk used as fuel for cooking in households

Mbassi Josiane Emilie Germaine
  • Mbassi Josiane Emilie Germaine
  • Food Technology Laboratory, Institute of Agricultural Research for Development (IRAD), P. O. Box 2123 Yaoundé, Cameroon.
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Sali Atanga Ndindeng
  • Sali Atanga Ndindeng
  • Africa Rice Center, M'bé Research Station, Bouake, 01BP 2551, Côte d’Ivoire.
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Achu Mercy Bih Loh
  • Achu Mercy Bih Loh
  • Department of Biochemistry, Faculty of Sciences, University of Yaoundé I, P. O. Box 812, Cameroon.
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Dimo Théophile
  • Dimo Théophile
  • Laboratory of Animal Physiology, Department of Animal Biology, University of Yaoundé I- P. O. Box 812, Cameroon.
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Mbacham Fon Wilfred
  • Mbacham Fon Wilfred
  • Department of Biochemistry, Faculty of Sciences, University of Yaoundé I, P. O. Box 812, Cameroon.
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  •  Received: 18 January 2020
  •  Accepted: 19 March 2020
  •  Published: 31 May 2020


This study meant to assess the toxicological impact of nourishment cooked or water overflowed with a fan-helped top-lit-updraft rice husk fuelled gasifier stove named Paul Olivier 150 (PO150). Refined water was bubbled for 1 h utilizing this stove in an opened pot and shut room. This water was then cooled to room temperature before being managed to the rodents with body loads going from 70 to 110 g. Two kinds of tests were performed: Acute and sub-chronic toxicity tests. For the acute toxicity study, an extraordinary portion of 2 ml/100 g body weight (BW) of bubbled water was managed orally to the rodents. The creatures were watched for harmful indications and mortality day by day for 14 days. In a sub-chronic toxicity study, the bubbled water, at dosages of 0.5, 1 and 2 ml/100 g BW were orally managed day by day for 28 days to rodents. Following 28 days, the rodents were yielded, Blood tests were gathered for hematological, biochemical and histological assessment. The control rodents were managed in refined water. The example of refined water overflowed with rice husk fuelled gasifier stove indicated no proof of single-portion toxicity (2 ml/100g) when studying acute toxicity. For the sub-chronic toxicity study, bubbled water at dosages of 0.5, 1 and 2 ml/100 g indicated huge contrast in certain parameters, for example, creatinine in guys (71.81 mg/dL), uric corrosive (2.75 mg/dL) and complete bilirubin (0.08 mg/dL), monocytes (0.49 103/µL)and granulocytes in females (2.70 103/µL) contrasted with the control gathering (64.16 mg/dL, 2.25 mg/dL, 0.19 mg/l, 0.37 103/µL and 1.80 103/µL for every parameter separately) however, the information did not ascend to the level for the responses to be viewed as a poisonous impact. These demonstrated that cooking in an open pot with a rice husk fuelled PO150 gasifier stove doesnot cause toxicity at the dosages considered.
Key words: Acute toxicity, sub-chronic toxicity, biochemical analysis, hematological parameters, histopathology, rice husk.