Crude and pure extracts of Tylophora indica were investigated in view of antifeedant, antibacterial and antifungal properties. Leaf crude extract showed more antifeedant activity than stem and root against Spodoptera litura, a polyphagous pest on wide ranging crops. Among the pure compounds isolated, Tylophorine showed the highest antifeedant activity followed by Septicine, O-Methyl Tylophorinidine and simple aliphatic acid. Similarly the crude extracts of leaf has exhibited higher antibacterial activity than root and shoot against Basillus subtilis, Staphylococcus aureus, Mycrococcus luteus and P. aergenosa. In contrast, Escherichia coli was not inhibited even at higher concentrations of either crude extracts or extracted pure compounds of T. Indica. Pure compounds displayed strong antibacterial activity at lower concentrations in all tested bacterial strains except E. coli. While all the crude and pure compounds showed antifungal activity against Aspergillus niger, Aspergillus fumigatus and Trichoderma viridae, the pure compounds had strong antifungal activity compared to crude extracts.
Key words: Tylophora indica, antifeedant activity, antimicrobial activity.