African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 381

Full Length Research Paper

Partial purification of invertase from Momordica charantia (bitter melon) by three phase partitioning (TPP) method

Nihan Kübra Belligün
  • Nihan Kübra Belligün
  • Department of Chemistry, Faculty of Arts and Sciences, Osmaniye Korkut Ata University, 80000 Osmaniye, Turkey.
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Burcu Saygıdeğer Demir
  • Burcu Saygıdeğer Demir
  • Department of Chemistry, Faculty of Arts and Sciences, Osmaniye Korkut Ata University, 80000 Osmaniye, Turkey.
  • Google Scholar


  •  Received: 04 March 2019
  •  Accepted: 25 April 2019
  •  Published: 30 June 2019

Abstract

The single step three-phase partitioning (TPP) method was evaluated for the purification of invertase from Momordica charantia. Optimum conditions for TPP method, that is, 70% ammonium sulfate saturation, 1:0.5 t-butanol ratio and 30 min incubation time lead to 20.28% efficiency and 10.48 fold partial purification. Total protein decreased from 34.86 mg to 0.68 mg in the homogenate, and the specific activity of the enzyme increased from 21.67 to 227.02 U/mg protein. The purified enzyme showed maximum activity in 0.3 M pH 5.0 buffer at 50°C. In conclusion, the optimized single step TPP method revealed better purification.

 

Key words: Three-phase partitioning (TPP), invertase, Momordica charantia, enzyme purification.