African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 403

Article in Press

Phytochemical and biological investigations of extracts from the roots of Cocos nucifera L. (Arecaceae) and Carica papaya L. (Caricaceae), two plants used in traditional medicine

LABIOCA- Université Joseph Ki-Zerbo and MEPHATRA/PH-IRSS (Institut de recherche en Sciences de la santé)

  •  Received: 21 November 2020
  •  Accepted: 20 January 2021
Cocos nucifera L. (Arecaceae) and Carica papaya L. (Caricaceae) are two plants used to treat several human pathologies such as oxidative stress and inflammatory diseases. The aim of this study was to assess anti-inflammatory activity, antioxidant activity and acute oral toxicity, of aqueous and methanolic extracts from Cocos nucifera L. and Carica papaya L. using appropriate experimental models. The acute oral toxicity test of extracts on mice was evaluated using Economic Cooperation and Development (OECD) guidelines 423. The capacity of extracts to inhibit key inflammation enzymes such as 15-lipoxygenase, phospholipase A2, cyclooxygenases 1 and 2 was evaluated in vitro. The antioxidant activity was assessed in vitro using four methods (inhibition of lipid peroxidation, FRAP, DPPH and ABTS tests). The phytochemical screening showed the presence of sterols and triterpenes, saponins, flavonoids, phenolics and tannins. Methanolic extract from Cocos nucifera exhibited higher values of 855.06 ± 1.71 mg GAE/ g, 418.22 ± 1.92 mg CE / g, 19.31 ± 0.6 mg GAE / g, 20.6 ± 0.36 mg QE / g dry extract respectively for phenolics, condensed tannins, hydrolysable tannins and flavonoid content. The extracts demonstrated antioxidant capacity, potential to inhibit pro-inflammatory enzymes and a lethal dose (LD50) was estimated to 5000 mg/kg b.w. The results of this study constituting a solid scientific basis that can justify the traditional uses of these plants.

Keywords: Cocos nucifera, Carica papaya, phytochemistry, acute oral toxicity, antioxidant, anti-inflammatory