African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 399


Glycated proteins: Clinical utility and analytical approaches

Yoseph Cherinet Megerssa
  • Yoseph Cherinet Megerssa
  • Department of Biomedical Sciences, College of Veterinary Medicine and Agriculture, Addis Ababa University, Ethiopia
  • Google Scholar
Demo Yemane Tesfaye
  • Demo Yemane Tesfaye
  • Department of Medical Laboratory Science, College of Medicine and Health Sciences, Hawassa University, Ethiopia
  • Google Scholar

  •  Received: 18 September 2014
  •  Accepted: 30 January 2015
  •  Published: 28 February 2015


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Brown B, Rashid I, Reyk D, Davies M (2007). Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages. Fed. Euro. Biochem. Soc. J. 274:1530-1541



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Cohen M., Clements R.(1999). Measuring glycated proteins: Clinical and methodological aspects. Diabet. Technol. Ther. 1(1):57-70.



Deric M, Stokic E, Vuckovic B, Kojic-Damjanov S, Cabarkapa V (2006). Lipids and atherosclerosis. Jugoslov Med. Biochem. 25:325-333



Epinex diagnostics Inc. (2008). Glycated albumin and diabetes monitoring. Corporate Information Series. 2:8-12.



Galle J, Wanner C (1999). Modification of lipoproteins in uremia: Oxidation, glycation and carbamoylation. Miner Electrol. Metab. 25:263-268



Graier WF, Kostner GM (1997). Glycated low density lipoprotein and atherogenesis. The missing link between diabetes mellitus and hypercholesterolemia. Eur. J. Clin. Invest. 27:457-459.



Hattori Y, Suzuki M, Hattori S, Kasai K (2002). Vascular smooth muscle cell activation by glycated albumin. Hypertension 39:22-28.



Hedrick C, Thorpe S, Fu M, Herper C, Yoo J, Kim S, Wong H, Peters A, Harper C (2000). Glycation impairs high-density lipoprotein function. Diabetologia 43:312-320.



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Inaba M, Okuno S, Kumeda Y, Yamada S, Imanishi Y, Tabata T, Okamura M, Okada S, Yamakawa T, Ishimura E, Nishizawa Y (2007). Glycated albumin is a better glycemic indicator than glycated hemoglobin values in hemodialysis patients with diabetes: effect of anemia and erythropoietin injection. J. Am. Soc. Nephrol. 18:896-903.



Jeppsson J, Kobold U, Barr J, Finke A, Wieland H, Hoshino T, Miedema K, Mosca A, Mauri P, Paroni R, Thienpont L, Umemoto M, Weykamp C (2002). Approved IFCC reference method for the measurement of HbA1c in human blood. Clinical Chemistry and Laboratory Medicine. 40(1):78-89.



John W (2003). Hemoglobin A1c: Analysis and Standardization, Clinical Chemistry & Laboratory Medicine. 41(9):1199-1212



Johnson RN, Metcalf PA, Baker JR (1983). Fructosamine: A new approach to the estimation of serum glycosyl protein. An index of diabetic control. Clin Chem Acta.127:87-95.



Klonoff D (2000). Serum fructosamine as a screening test for type 2 diabetes. Diabet. Technol. Ther. (4):537-539.



Kobayashl K, Ogasahara N, Sekoguchl T, Kimura M, Tanluchi K, Matsuoka A (1990). Agarose gel electrophoresis with nitrobluetetrazolium coloration for determination of glycated lipoproteins in serum from diabetic. Clin. Chem. 36(1):65-69.



Koga M, Murai J, Saito H, Kasayama S (2010). Glycated albumin and glycated hemoglobin are influenced differently by endogenous insulin secretion in patients with type 2 diabetes. Diabet. Care 33:270-272.



Kouzuma T, Uemastu Y, Usami T, Imamura S (2004). Study of glycated amino acid elimination reaction for an improved enzymatic glycated albumin measurement method. Clinica Chimica Acta. 346:135-143.



Kouzuma T, Usami T, Yamakoshi M, Takahashi M, Imamura S (2002). An enzymatic method for the measurement of glycated albumin in biological samples, Clinica Chimica Acta. 324:61-71



Kumeda Y, Inaba M, Shoj S, Ishimura E, Inariba H, Yabes S, Okamura M, Nshizawa Y (2008). Significant correlation of glycated albumin, but not glycated hemoglobin, with arterial stiffening in haemodialysis patients with type 2 diabetes. Clin. Endocrinol. 69:556-561.



Lacinová K, Eckhardt A, Pataridis S, Sedláková P, Mikšík I. (2010). Glycation of proteins: their analysis and physiological aspects, Advances in Molecular Mechanisms and Pharmacology of Diabetic Complications. 17-38, ISBN: 978-81-7895-499-8



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Megerssa YC, Gebre MW, Birru SK, Goshu AR, Tesfaye DY (2013). Prevalence of undiagnosed diabetes mellitus and its risk factors in selected institutions at Bishoftu Town, East Shoa, Ethiopia. J. Diabet. Metab. S12:008.



Miller A, Adeli K (2008). Diatery fructose and the metabolic syndrome. Curr. Opin. Gasteroentrol. 24:204-209.



Mula-Abed W, Hanna B (2001). Measurement of serum fructosamine as an index of glycated protein in patients with nephrotic syndrome and with chronic liver diseases bahrain. Med. Bull. 23(4):169-174.



Nakayama M, Lzumi G, Nemoto Y, Shibata K, Hasegawa T, Numata M, Wang Kengmin, Kawaguchi Y, Hosoya T. (1999). Suppression of Nε-(carboxymethyl) lysine generation by the antioxidant N-acetylcysteine, International Society for Peritoneal Dialysis 19:207-210.



Nitin S. (2010). HbA1c and factors other than diabetes mellitus affecting it. Singapore Med. J. 51(8):616-622.



Okumura A, Mitamura Y, Namekata K, Nakamura K, Harada C, Harada T (2007). Glycated albumin induces activation of activator protein-1 in retinal glial cells. J. Ophthalmol. 51:231-243.



Pu LJ, Lu L, Shen WF, Zhang Q, Zhang RY., Zhang JS., Hu J., Yang ZK., Ding FH., Chen QJ, Shen J, Fang DH, Lou S (2007). Increased serum glycated albumin level is associated with the presence and severity of coronary artery disease in type 2 diabetic patients. Circ. J. 71:1067-1073.



Reddy SA, Sachan A., Srinivasa RP, Mohan A (2012). Clinical applications of glycosylated hemoglobin. J. Clin. Sci. Res.2:22-33.



Schleicher E, Wieland OH (1986). Kinetic analysis of glycation as a tool for assessing the half-life of proteins. Biochim. Biophys. Acta 875:65-71.



Schleicher ED, Olgemoller B, Wiedenmann E, Gerbitz ED (1993). Specific glycation of albumin depends on its half-life. Clin. Chem. 39:625-628.



Scleiche ED, Vogt BW (1990). Standardization of serum fructosamine assays. Clin. Chem. 36:136-139.



Singh NR, Rondeau P, Hoareau L, Bourdon E (2007). Identification of preferential protein targets for carbonylation in human mature adipocytes treated with native or glycated albumin. Free Rad. Res. 41(10):1078-1088.



Sultanpur C, Kumar D (2010). Comprehensive review on HbA1c in diagnosis of diabetes mellitus. Int. J. Pharmaceut. Sci. Rev. Res. 3(2):119-122



Tahara Y, Shima K (1995). Kinetics of HbA1c, glycated albumin, and fructosamine and analysis of their weight functions against preceding plasma glucose level. Diabetes Care 18:440-447.



Takahashi S, Uchino H, Shimizu T, Kanazawa A, Tamura Y, Sakai K, Watada H, Hirose T, Kawamori R, Tanaka Y (2007). Comparison of glycated albumin (GA) and glycated hemoglobin (HbA1c) in type 2 diabetic patients: usefulness of GA for evaluation of short-term changes in glycemic control. Endocr. J. 54:139-144.



Tanaka A (2001). Assay for glycated lipoproteins in atherosclerosis. Methods in Molecular Medicine 52:105-111



Tanaka A, Yui K, Tomie N (2006). New assay for glycated lipoproteins by high-performance liquid chromatography, Annals New York academy of Sciences. 811(1):385-394.



Tesfaye DY, Kinde S, Medhin G, Megerssa YC, Tadewos A, Tadesse E, Shimelis T (2014). Burden of metabolic syndrome among HIV-infected patients in Southern Ethiopia. Diabetes & Metabolic Syndrome: Clinical Research & Reviews. 8:102-107.



Veiraia A, Wales P (2005). Hyperglycemia, lipoprotein glycation and vascular disease, Angiology 56:431-438.



Xua Y, Wub X, Liua W, Lin X, Chen J, He R (2002). A convenient assay of glycoserum by nitrobluetetrazolium with iodoacetamide, Clinica Chimica Acta.325:127-131.