August 2007

Suppression-subtractive hybridization: A rapid and inexpensive detection methodology for up-regulated Perkinsus olseni genes

  A cDNA forward subtraction library was constructed from the mollusc parasite Perkinsus olseni exposed to hemolymph from its natural host, the clam Ruditapes decussatus, and two different methodologies were used to unravel different non-redundant contigs. Our results demonstrated that screening of the non-enriched direct cDNA subtractive library (Dfsl) was the most efficient and least time-...

Author(s): Ascenso R. M. T., Leite R. B., Afonso R., Cancela M. L.

August 2007

Analysis of phenolic compounds in Ugandan sweet potato varieties (NSP, SPK AND TZ)

      Sweet potato varieties: Nsp, Tz and Spk obtained from Uganda were used in this study. Several phenolic compounds were identified on the basis of their mass spectra in full scan mode and the pattern of their fragmentation. The major compounds found were caffeoylquinic acid, dicaffeoylquinic acid, coumaroylquinic acid,...

Author(s): Agnes M. Nandutu, Mike Clifford and Nazlin K. Howell

August 2007

Altered plasma hexose sugar metabolism in sickle cell anaemia

  Theoretical suggestion that the metabolism and plasma levels of some hexoses (glucose and fructose) in sickle cell anaemia could be altered was investigated.  The levels were determined for 35 normal (HbAA) and for the three sickle cell states (32 HbAS; 35 HbSS, and 33 HbSS-in-crisis, HbSSc). The enzymatic glucose-oxidase method was employed in glucose estimation. The reaction of fructose with...

Author(s): Chidi G. Osuagwu and Chika U. Mbeyi