African Journal of
Cellular Pathology

OFFICIAL PUBLICATION OF THE SOCIETY FOR CELLULAR PATHOLOGY SCIENTISTS OF NIGERIA
  • Abbreviation: Afr. J. Cell. Path
  • Language: English
  • ISSN: 2449-0776
  • DOI: 10.5897/AJCPath
  • Start Year: 2013
  • Published Articles: 80

IMMUNOHISTOCHEMICAL EXPRESSION OF p16(INK4A) PROTEIN IN CERVICAL DYSPLASIA AND CARCINOMA IN PATIENTS ATTENDING FEDERAL TEACHING HOSPITAL, GOMBE, NIGERIA

Umar A
  • Umar A
  • Faculty of Medical Laboratory Science, Usmanu Danfodiyo University Sokoto, Nigeria.
  • Google Scholar
Avwioro OG
  • Avwioro OG
  • Faculty of Science, Delta State University, Abraka, Nigeria.
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Muhammad AT
  • Muhammad AT
  • Faculty of Medical Laboratory Science, Usmanu Danfodiyo University Sokoto, Nigeria.
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Mohammed I
  • Mohammed I
  • Faculty of Medical Laboratory Science, Usmanu Danfodiyo University Sokoto, Nigeria.
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Mohammed MO
  • Mohammed MO
  • Faculty of Medical Laboratory Science, Usmanu Danfodiyo University Sokoto, Nigeria.
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Ibrahim KK
  • Ibrahim KK
  • Faculty of Medical Laboratory Science, Usmanu Danfodiyo University Sokoto, Nigeria.
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Sahabi SM
  • Sahabi SM
  • Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria.
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Abdullahi K
  • Abdullahi K
  • Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria.
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Mohammed SA
  • Mohammed SA
  • Federal Teaching Hospital, Gombe, Nigeria.
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Ajayi AS
  • Ajayi AS
  • Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria.
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  • Article Number - 1C4D07365710
  • Vol.7(6), pp. 41-46 , December 2016
  •  Received: 23 July 2016
  •  Accepted: 23 October 2016
  •  Published: 31 December 2016

Abstract

Aim: This study was conducted to determine and compare the immunohistochemical expression of p16(INK4a) in cervical dysplasia and carcinoma in Gombe, Nigeria.

Materials: Seventy eight cases of cervical neoplasm; comprising 17 cases of cervical intraepithelial neoplasia (CIN), 53 cases of squamous cell carcinoma (SCC), 6 cases of adenocarcinoma and 2 cases of adenosquamous carcinoma. All samples were stained using p16(INK4a) Rabbit monoclonal anti-CDKN2A/p16(INK4a) antibody.

Results: The p16(INK4a) expression was graded as negative, focal and diffuse positivity. The study showed p16(INK4a) expression in CIN I, CIN II and CIN III as 0%, 80.0% and 83.3% respectively. Majority of SCC (88.7%), adenocarcinoma (83.3%) and adenosquamous carcinoma (100%) showed p16(INK4a) expression.

Conclusion: Immunohistochemical detection of p16(INK4a) can be a useful diagnostic marker for all degrees of cervical dysplasias and carcinomas.

Key words: Dysplasia, Carcinoma, Immunohistochemistry, HPV, p16, Neoplasia,