African Journal of
Environmental Science and Technology

  • Abbreviation: Afr. J. Environ. Sci. Technol.
  • Language: English
  • ISSN: 1996-0786
  • DOI: 10.5897/AJEST
  • Start Year: 2007
  • Published Articles: 922

Full Length Research Paper

Biodegradation of pyrene by a mixed culture isolated from hydrocarbon-polluted soil

Baba Shehu Umar Ibn Abubakar
  • Baba Shehu Umar Ibn Abubakar
  • Department of Civil and Water Resources Engineering, Faculty of Engineering, University of Maiduguri, P. M. B. 1069, Maiduguri, Borno State, Nigeria.
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Norhafizah Abdullah
  • Norhafizah Abdullah
  • Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia.
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Azni Idris
  • Azni Idris
  • Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia.
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Mohamad Pauzi Zakaria
  • Mohamad Pauzi Zakaria
  • Department of Environmental Sciences, Faculty of Environmental Studies, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia.
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Mohd Yunus Shokur
  • Mohd Yunus Shokur
  • Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia.
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  •  Received: 07 September 2018
  •  Accepted: 06 November 2018
  •  Published: 31 January 2019

Abstract

This paper presents the biodegradation of pyrene (C16H10), a four-benzene-ring polycyclic aromatic hydrocarbon (PAHs) using a mixed culture of bacteria. The mixed culture was enriched from hydrocarbon-polluted soil sample, using a plate incubated with a thin film of pyrene. The isolates were identified biochemically as Bacillus cereus and Enterobacter aerogenes. The mixed culture bacteria degraded approximately 90% of the pyrene in aqueous medium within six days of incubation. The assessment of other environmental factors necessary for the activity of the mixed culture revealed that the mixed culture grows well between pH of 5.0-7.0 and temperatures of between 20-30°C. From the results of this trial therefore, it is suggested that the mixed culture has good potential for bioremediation applications.

 

Key words: Pyrene, biodegradation, mixed culture, polycyclic aromatic hydrocarbon (PAHs), environmental factors.