African Journal of
Food Science

  • Abbreviation: Afr. J. Food Sci.
  • Language: English
  • ISSN: 1996-0794
  • DOI: 10.5897/AJFS
  • Start Year: 2007
  • Published Articles: 790

Full Length Research Paper

Indicator microorganisms, Salmonella, Listeria monocytogenes, Staphylococcal enterotoxin, and physicochemical parameters in requeson cheese

Angélica Villarruel-López
  • Angélica Villarruel-López
  • Laboratorio de Microbiología Sanitaria, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Marcelino García Barragán No. 1421, 44430 Guadalajara, Jalisco, México.
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Javier Castro-Rosas
  • Javier Castro-Rosas
  • Área Académica de Química, Ciudad del Conocimiento, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo Km 4.5, 42183 Pachuca, Hidalgo, México.
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Carlos A. Gómez-Aldapa
  • Carlos A. Gómez-Aldapa
  • Área Académica de Química, Ciudad del Conocimiento, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo Km 4.5, 42183 Pachuca, Hidalgo, México.
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Karla Nuño
  • Karla Nuño
  • Departamento de Ciencias de la Salud, Centro Universitario de Tonalá, Universidad de Guadalajara, Av. Periférico Norte No. 555, 48525 Tonalá, Jalisco, México.
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Ma. R. Torres-Vitela
  • Ma. R. Torres-Vitela
  • Laboratorio de Microbiología Sanitaria, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Marcelino García Barragán No. 1421, 44430 Guadalajara, Jalisco, México.
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Nanci E. Martínez-Gonzáles
  • Nanci E. Martínez-Gonzáles
  • Laboratorio de Microbiología Sanitaria, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Marcelino García Barragán No. 1421, 44430 Guadalajara, Jalisco, México.
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Luz E. Garay-Martínez
  • Luz E. Garay-Martínez
  • Laboratorio de Microbiología Sanitaria, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Marcelino García Barragán No. 1421, 44430 Guadalajara, Jalisco, México.
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  •  Received: 03 June 2016
  •  Accepted: 12 July 2016
  •  Published: 30 September 2016

Abstract

Requeson cheese is an artisanal dairy product widely consumed in Latin America. However, no data is available on its microbiological and physicochemical quality. Eighty-four samples were collected. Identification and enumeration of indicator microorganisms and pathogens were done by conventional culture methods, meanwhile staphylococcal enterotoxin by immunoassay. Bromatological parameters were measure collection day. Indicator and pathogenic microorganisms per samples were aerobic mesophilic bacteria (n = 100), coliform bacteria (n = 100), molds-yeasts (n = 70), Staphylococcus aureus (n = 74), and Salmonella (n = 3). Neither Listeria monocytogenes nor Staphylococcal enterotoxin was detected. Temperature ranged from 3 to 19°C, pH from 5.1 to 6.9, acidity from 0.1 to 0.7%, and sodium from 290 to 3970 mg/100 g. Protein content ranged from 2.1 to 13% and lipids from 4.1 to 13%. Starch as adulterant was present in 56 samples.

Key words: Indicator microorganism, Salmonella, Listeria monocytogenes, Staphylococcal enterotoxin, macronutrients, sodium.