African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4962

Full Length Research Paper

Anti-quorum quenching activity of methyl gallate isolated from galls of Guiera senegalensis J. F. Gmel (Combretaceae)

Vincent Ouedraogo
  • Vincent Ouedraogo
  • Laboratory of Biochemistry and Chemistry Applied (LABIOCA), University of Ouaga I Professor Joseph Ki-Zerbo, 09 P. O. Box 848 Ouagadougou 09, Burkina Faso.
  • Google Scholar
Pierre Alexandre Eric Djifaby Sombié
  • Pierre Alexandre Eric Djifaby Sombié
  • National Center of Scientific and Technological Research, Institute of Environment and Agricultural Research, 01 P. O. Box 476 Ouagadougou 01, Burkina Faso.
  • Google Scholar
Moussa Compaoré
  • Moussa Compaoré
  • Laboratory of Biochemistry and Chemistry Applied (LABIOCA), University of Ouaga I Professor Joseph Ki-Zerbo, 09 P. O. Box 848 Ouagadougou 09, Burkina Faso.
  • Google Scholar
Martin Kiendrébéogo
  • Martin Kiendrébéogo
  • Laboratory of Biochemistry and Chemistry Applied (LABIOCA), University of Ouaga I Professor Joseph Ki-Zerbo, 09 P. O. Box 848 Ouagadougou 09, Burkina Faso.
  • Google Scholar


  •  Received: 21 January 2019
  •  Accepted: 17 April 2019
  •  Published: 28 April 2019

Abstract

Many pathogenic bacteria produce virulence factors controlled by a mechanism of regulation named quorum sensing (QS). Inhibition of bacterial QS system is a more recent therapeutic approach to counterbalance the emergence of multi-drug resistant bacteria. This study aimed to assess the abilities of methanol extract from Guiera senegalensis galls and its isolated compound methyl gallate to quench the quorum sensing system. Methanol extract from galls of G. senegalensis at the concentration of 100 µg/ml demonstrated significant inhibitory effect on pyocyanin and violacein production respectively in Pseudomonas aeruginosa PAO1 and Chromobacterium violaceum CV026. Colum chromatography and recycling High Performance Liquid Chromatography (HPLC) of methanol extract from galls of G. senegalensis led to the isolation of one active quorum quenching compound. Different spectroscopic methods (MS and NMR) were used to elucidate the structure of this isolated compound as being the methyl gallate (MG). Methyl gallate at the final concentration of 12.5 µg/ml demonstrated good anti-QS activity by inhibiting violacein and pyocyanin production. Its low molecular weight and the capacity to interfere with the mechanism of QS make methyl gallate, an interesting candidate for development of drugs as an alternative to antibiotics to combat bacterial resistance.

 

Key words: Guiera senegalensis, methyl gallate, Pseudomonas aeruginosa PAO1, quorum sensing.