Abstract

The present of research work was focused on mesophilic soil samples from four different sites of Jaipur (The Pink City) in Rajasthan. Rajasthan is a semi arid region and famous for its sandy soil, heat and temperature which reach up to 50°C in summers. Twenty nine colonies were isolated from the soil samples on nutrient agar media and three distinct isolates were selected and characterized after analyzing coloration of colony formation and zone of inhibition around and appearance of the colony. On the basis of biochemical tests, the isolates are suspected to belong to the genera, Bacillus, Actinomycetes and Staphylococcus. Antibiotic activity of the isolates was studied against some common nosocomial infection causing pathogens and promising results were observed. Out of 29 colonies, only 3 colonies viz. S-IA, S-IIA and S-IIIC showed appreciable biological activity against the above mentioned 2 test pathogens E. coli and Pseudomonas aeruginosa. Further, inhibition zones were calculated in S-IA (IZ = 12 mm; AI = 1.5); S-II A (IZ = 42 mm; AI = 4.2) and S-III C (IZ = 12 mm; AI = 1.1).

Key words: Antimicrobial efficacy, nosocomial infections, mesophilic soil, metabolomes, Rajasthan.