African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5128

Full Length Research Paper

Antibiotic potentials and isolation of metabolomes from microorganisms of mesophilic soil of Rajasthan, India

Abhishek Sharma
  • Abhishek Sharma
  • Department of Microbiology, JECRC University, Plot No. IS-2036 to 2039, Sitapura Industrial Area Extn, Near Mahatma Gandhi Hospital, Ramchandrapura, Jaipur, Rajasthan 303905, India.
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Ekta Menghani*
  • Ekta Menghani*
  • Department of Biotechnology, JECRC University, Plot No. IS-2036 to 2039, Sitapura Industrial Area Extn, Near Mahatma Gandhi Hospital, Ramchandrapura, Jaipur, Rajasthan 303905, India.
  • Google Scholar


  •  Received: 06 December 2015
  •  Accepted: 06 September 2016
  •  Published: 28 February 2017

Abstract

The present of research work was focused on mesophilic soil samples from four different sites of Jaipur (The Pink City) in Rajasthan. Rajasthan is a semi arid region and famous for its sandy soil, heat and temperature which reach up to 50°C in summers. Twenty nine colonies were isolated from the soil samples on nutrient agar media and three distinct isolates were selected and characterized after analyzing coloration of colony formation and zone of inhibition around and appearance of the colony. On the basis of biochemical tests, the isolates are suspected to belong to the genera, Bacillus, Actinomycetes and Staphylococcus. Antibiotic activity of the isolates was studied against some common nosocomial infection causing pathogens and promising results were observed. Out of 29 colonies, only 3 colonies viz. S-IA, S-IIA and S-IIIC showed appreciable biological activity against the above mentioned 2 test pathogens E. coli and Pseudomonas aeruginosa. Further, inhibition zones were calculated in S-IA (IZ = 12 mm; AI = 1.5); S-II A (IZ = 42 mm; AI = 4.2) and S-III C (IZ = 12 mm; AI = 1.1).

Key words: Antimicrobial efficacy, nosocomial infections, mesophilic soil, metabolomes, Rajasthan.