African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5172

Full Length Research Paper

Recombinant Clone ABA392 protects laboratory animals from Pasteurella multocida Serotype B

Jamal Hussaini1, Nazmul M. H. M.1, Mahmood A. Abdullah2 and Salmah Ismail2
1Faculty of Medicine, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia. 2Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Email: [email protected]

  •  Accepted: 23 June 2011
  •  Published: 16 September 2011

Abstract

In this study the potential of the previously contracted recombinant clone ABA392 derived from Pasteurella multocida serotype B to protect laboratory animal against haemorrhagic septicaemia was determined. After reconfirmation of plasmid DNA size, its stability and pathological effect of the clone, passive mice protection test and active immunization was carried out. Pooled serum sample from rats vaccinated with live and killed form of the clone was administered to mice and provided 66% protection while active immunization with the recombinant clone conferred 83% immunity to mice when challenged with lethal dose of P. multocida. ELISA results were positive for presence of antibody in serum of immunized mice. Sub-cloning of the insert ABA392 into an expression vector pQE32 was carried out to express its protein. It was found that the recombinant clone ABA392 is immunogenic and could be used as vaccine in future.

 

Key words: Recombinant clone, immunogenicity, Pasteurella multocida serotype B.