African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4955

Full Length Research Paper

Self and cross incompatibility traits analysis in some diseases tolerant almond genotypes by PCR

Yavar Sharafi1*, Magsood Kafshnochi2 and Amir Mohammad Naji3        
1Department of Horticulture, Faculty of Agriculture, Shahed University- Opposite Holy Shrine of Imam Khomeini- Khalij Fars Expressway- Tehran- Iran. 2Departement of Basic Sciences, science Paradise, University of Tehran, Tehran- Iran 3Department of Plant Breeding and Agronomy, Faculty of Agriculture Shahed University- Opposite Holy Shrine of Imam Khomeini- Khalij Fars Expressway- Tehran- Iran.
Email: [email protected]

  •  Accepted: 30 November 2011
  •  Published: 16 January 2012

Abstract

Almond is one of the oldest nut crops in the world and has a main problem called self-incompatibility. Identification of S alleles in almond cultivars and genotypes is essential for breeders and growers. The aim of this study was to identify S alleles pattern in 30 late bloom and resistant to aphid, mite, Monilia laxa and "Polystigma occharaceum" fungus almond genotypes from different geographical regions of East-Azerbaijan Iran. Most of the S alleles were amplified using Pru-C2/Pru-C5R primer pair and size of alleles ranged between 2000-220 bp in genotypes. Considering all of the S alleles amplified using 5 primer pairs in thirty numbers of the genotypes two S alleles were detected and showed their fully self-incompatibility pattern, in 10 genotypes only one of the S alleles were.

 

Key words: Almond, S allele, specific primers, self-incompatibly, inter-incompatibility.

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