African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4973

Full Length Research Paper

Antibiotic resistant phenotypes of Staphylococcus aureus isolated from fresh and fermented milk in parts of Nasarawa State, Nigeria

Aliyu Y.
  • Aliyu Y.
  • Department of Science Laboratory Technology, School of Applied Sciences, Federal Polytechnic, P.M.B. 001, Nasarawa, Nasarawa State, Nigeria.
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Abdullahi I. O.
  • Abdullahi I. O.
  • Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Nigeria.
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Whong C. M. Z.
  • Whong C. M. Z.
  • Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Nigeria.
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Olayinka B. O.
  • Olayinka B. O.
  • Department of Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria, Nigeria.
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  •  Received: 22 July 2019
  •  Accepted: 20 August 2019
  •  Published: 31 August 2019

Abstract

This work was aimed at determining the occurrence and antibiogram of Staphylococcus aureus isolated from fresh and fermented milk samples in parts of Nasarawa State, Nigeria. A total of 180 samples comprising of fresh raw milk, bulk milk, nono, and kindirmo were collected over a period of 6 months (May to October, 2017). Standard microbiological procedures were employed in the isolation, identification, characterisation, and determination of the antibiogram of S. aureus from the milk samples. Characterisation of the S. aureus isolates was by morphological, biochemical characteristics using conventional methods, Microgen® STAPH-ID kits. Confirmed isolates were tested for susceptibility or resistance to a panel of 11 commonly used antibiotics using the agar disc diffusion technique. Out of the 180 milk samples examined, 9 S. aureus were isolated giving a prevalence of 5.0%. The occurrence of S. aureus was higher in nono (12.1%) and kindirmo (10.6%) than in fresh raw milk (5.9%). The high occurrence of S. aureus in nono disproved the assertion that fermented foods are not good media for the survival and growth of S. aureus. The antibiotic susceptibility profile of the S. aureus isolates indicated all of the nine isolates were completely resistant to cefoxitin, ampicillin, and amoxicillin/clavulanic acid. The isolates were moderately resistant to erythromycin (22.2%), sulphamethoxazole/trimethoprim (22.2%), and tetracycline (44.4%). Five antibiotic resistance patterns were recorded among the isolates. All of the isolates had a multiple antibiotics resistance (MAR) index of 0.3 and above, an indication of possible antibiotic misuse in the areas studied.

 

Key words: Milk, Staphylococcus aureus, antibiotic resistant phenotypes, Nasarawa State, Nigeria.