African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5123

Full Length Research Paper

Monitoring of physicochemical and microbiological parameters during nonnonkoumou (artisanal curdled milk) production in Daloa, Côte d’Ivoire

ASSOHOUN-DJENI Nanouman Marina Christelle
  • ASSOHOUN-DJENI Nanouman Marina Christelle
  • Université Jean Lorougnon Guédé, Unité de Formation et de Recherche en Agroforesterie, Laboratoire de microbiologie, Bio-industrie et Biotechnologie, BP150 Daloa, Côte d’Ivoire.
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ATTIEN Yao Paul
  • ATTIEN Yao Paul
  • Université Jean Lorougnon Guédé, Unité de Formation et de Recherche en Agroforesterie, Laboratoire de microbiologie, Bio-industrie et Biotechnologie, BP150 Daloa, Côte d’Ivoire.
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KOUASSI Kra Athanase
  • KOUASSI Kra Athanase
  • Université Jean Lorougnon Guédé, Unité de Formation et de Recherche en Agroforesterie, Laboratoire de microbiologie, Bio-industrie et Biotechnologie, BP150 Daloa, Côte d’Ivoire.
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KOUASSI Kouassi Clément
  • KOUASSI Kouassi Clément
  • Université Jean Lorougnon Guédé, Unité de Formation et de Recherche en Agroforesterie, Laboratoire de microbiologie, Bio-industrie et Biotechnologie, BP150 Daloa, Côte d’Ivoire.
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KOUAME Aya Bah Marie-Ange Christelle
  • KOUAME Aya Bah Marie-Ange Christelle
  • Université Jean Lorougnon Guédé, Unité de Formation et de Recherche en Agroforesterie, Laboratoire de microbiologie, Bio-industrie et Biotechnologie, BP150 Daloa, Côte d’Ivoire.
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DJENI N’dédé Théodore
  • DJENI N’dédé Théodore
  • Université Nangui Abrogoua, Unité de Formation et de Recherche en Sciences et Technologies des Aliments, Laboratoire de Biotechnologie et microbiologie des aliments, 02 BP 801 Abidjan 02 Côte d’Ivoire.
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KONATE Ibrahim
  • KONATE Ibrahim
  • Université Jean Lorougnon Guédé, Unité de Formation et de Recherche en Agroforesterie, Laboratoire de microbiologie, Bio-industrie et Biotechnologie, BP150 Daloa, Côte d’Ivoire.
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  •  Received: 15 February 2021
  •  Accepted: 31 March 2021
  •  Published: 30 June 2021

Abstract

This study aimed to study physicochemical and microbiological parameters during nonnonkoumou production. A total of 15 samples were analyzed during this study at a rate of 3 samples per fermentation time (T0 h, T6 h, T12 h, T18 h and T24 h). The physicochemical analyses showed a drop in pH (from 7.03 ± 0.028 to 4.59 ± 0.021) during the 24 h of fermentation. The titratable acidity increased from 0.30 ± 0.014% at the start of fermentation to reach the maximum value of 0.88 ± 0.056% at 24 h of fermentation. The sugar level of fermenting milk samples decreased from 10.05 ± 0.071% at the start of fermentation, to 5.15 ± 0.071% at the end of fermentation. The density results showed an addition of water to the milk used for nonnonkoumou production. Microbiological analyses showed a similar growth of lactic acid bacteria and yeasts and molds, but yeasts and molds were absent at the start of fermentation. Aerobic mesophile flora count reached maximum value (7.56 ± 0.81 Log CFU/ml) at 12 h of fermentation. The coliform count increased up to 12 h of fermentation before decreasing and disappearing at the end of fermentation.

 

Key words: Milk, nonnonkoumou, fermentation, contamination, physicochemical and microbiological analyses.