African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5173

Full Length Research Paper

Isolation and in-vitro assessment of antagonistic activity of Trichoderma spp. against Magnaporthe oryzae Longorola strain causing rice blast disease in Mali

Mahamadou Diarra
  • Mahamadou Diarra
  • LaboREM-Biotech, Faculty of Sciences and Techniques, University of Sciences, Techniques and Technologies of Bamako, Bamako, BP E 3206 Mali.
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Adounigna Kassogué
  • Adounigna Kassogué
  • LaboREM-Biotech, Faculty of Sciences and Techniques, University of Sciences, Techniques and Technologies of Bamako, Bamako, BP E 3206 Mali.
  • Google Scholar
Amadou Hamadoun Babana
  • Amadou Hamadoun Babana
  • LaboREM-Biotech, Faculty of Sciences and Techniques, University of Sciences, Techniques and Technologies of Bamako, Bamako, BP E 3206 Mali.
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Oumarou Hamadoun
  • Oumarou Hamadoun
  • Centre Régional de Recherche Agronomique (CRRA), Institut d’Économie Rurale, Sikasso, Mali.
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Fousseyni Cissé
  • Fousseyni Cissé
  • Centre Régional de Recherche Agronomique (CRRA), Institut d’Économie Rurale, Sikasso, Mali.
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Abdoulaye Hamadoun
  • Abdoulaye Hamadoun
  • Direction Nationale de l’Institut d’Économie Rurale (IER), Bamako, Mali.
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  •  Received: 03 January 2021
  •  Accepted: 26 January 2022
  •  Published: 28 February 2022

Abstract

Pyricularia oryzea (Magnaporthe oryzae) causes blast diseases in rice (Oryza sativa) in Mali. The losses could reach 90% of production during rainy weather conditions. Isolation and characterization of M. oryzae and Trichoderma species were carried out to assess the importance and distribution of the pathogen and antagonist Trichoderma species in rice fields in Sikasso (Mali), and select, in vitro, Trichoderma species with high pathogen biocontrol activity. In the pathogen isolation, only one isolate of M. oryzae were obtained, while 12 Trichoderma isolates were obtained. In the fungal growth tests three isolates of Trichoderma: Trichoderma harzianum S31, T. harzianum S32, and T. harzianum S33 highly inhibited the growth of the pathogen with a coefficient of antagonism of 0.55, 0.71 and 0.78 respectively. These isolates were selected for further greenhouse and field tests.

 

Keys words: Rice blast disease, Trichoderma, Magnaporthe oryzae, Pyricularia oryzea, antagonism, Oryza sativa, Mali.