African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5056

Full Length Research Paper

Prevalence of Listeria spp. in fish obtained from Urmia fish markets

Rojan Modaresi1, Karim Mardani1*, Amir Tukmechi2 and Abdolghaffar Ownagh3
  1Division of Molecular Epidemiology, Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, West Azarbaijan, Iran.   2Department of Pathobiology and Quality Control, Artemia and Aquatic Animals Research Institute, Urmia University, Urmia, West Azarbaijan, Iran.   3Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, West Azarbijan, Iran.
Email: [email protected]

  •  Accepted: 17 October 2011
  •  Published: 16 December 2011

Abstract

 

The present study was conducted to determine the prevalence of Listeria spp. in fish obtained from Urmia fish markets. A number of 194 fish comprising Oncorhynchus mykiss (n=42), Sander lucioperca (n=38), Cyprinus carpio (n=30), Hypophthalmiachthys molitrix (n=48), Abramis brama (n=12), Astacus leptodactylus (n=12) and Silurus glanis(n=12) were obtained from different fish markets of Urmia from June 2009 to February 2010. Listeria isolation was performed in two stages including enrichment in cold and selective plating. After colony formation, in order to confirming the genus of the Listeria, a fragment of Prs gene using Listeria genus specific primers was amplified from isolated bacteria using polymerase chain reaction (PCR). Results revealed that Listeria was isolated from 24 fish (12.37%). The highest prevalence of Listeria was observed in both A. brama and A. leptodactylus with 25%, while the lowest prevalence of Listeria was seen inS. lucioperca (9.7%). From the total of 24 Listeria isolates, five isolates (21%) were confirmed to be L. monocytogenes; seven isolates were L. ivonoi (29%) while L. Seeligeriwas not isolated from any examined fish. The study showed that L. monocytogenes and other Listeria species are common contaminant of fish obtained from Urmia fish markets, and this may pose serious public health implications.

 

Key words: Listeria, Listeria monocytogenes, fish, PCR, Urmia.