African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5037

Full Length Research Paper

Cytological changes in Solanum lycopersicum seedlings in response to some rhizopheric fungi

Ahmed Fouad Afifi
  • Ahmed Fouad Afifi
  • Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University, Roxy, Cairo 11757, Egypt.
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Ehab Abdel-Razik Kamel
  • Ehab Abdel-Razik Kamel
  • Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University, Roxy, Cairo 11757, Egypt.
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Amal Abdelsamei Elsaed
  • Amal Abdelsamei Elsaed
  • Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University, Roxy, Cairo 11757, Egypt.
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Mohamed Abdelaziz Foaad
  • Mohamed Abdelaziz Foaad
  • Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University, Roxy, Cairo 11757, Egypt.
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Eman Mohamed Fawzy
  • Eman Mohamed Fawzy
  • Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University, Roxy, Cairo 11757, Egypt.
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Heba Ibrahim Abo-Elmagd
  • Heba Ibrahim Abo-Elmagd
  • Preparatory Year and Supporting Studies, Department of Basic Sciences, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
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  •  Received: 10 February 2020
  •  Accepted: 07 April 2020
  •  Published: 31 July 2020

Abstract

Some rhizospheric fungi of Solanum lycopersicum were isolated from Cairo governorate and identified in Taxonomy Department, Ain-Shams University, Cairo City, Egypt. These isolated fungi were cultivated on soil extract medium enriched with 2% (w/v) glucose and 5% (w/v) yeast extract. The cytological effects of the metabolites of these fungi on the seeds of S. lycopersicum were examined after three different intervals (6, 12 and 24 h). These cytological effects include three parameters; chromosomal aberrations, nucleic acids content, protein banding patterns. Moreover, ochratoxin A was determined in the fungal filtrate of the most effective isolate using HPLC technique.

 

Key words: Rhizospere, fungi, Solanum lycopersicum, mitotic index, chromosomal aberrations, nucleic acids, protein patterns.