African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4889

Full Length Research Paper

Antimicrobial, antiparasitic and antiproliferative effects of the extract of Bacillus safensis SG-32 isolated from a Brazilian oil reservoir

Tiago Rodrigues e Silva
  • Tiago Rodrigues e Silva
  • Division of Microbial Resources, CPQBA UNICAMP, Campinas, Brazil.
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Eugenio Vaz dos Santos Neto
  • Eugenio Vaz dos Santos Neto
  • Petrobras R and D Center, Cidade Universitária, Block 7, Rio de Janeiro, RJ, CEP: 21949-900, Brazil.
  • Google Scholar
Ana Lucia Tasca Goiz Ruiz
  • Ana Lucia Tasca Goiz Ruiz
  • Division of Chemistry of Natural Products, CPQBA UNICAMP, Campinas, Brazil.
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Mary Ann Foglio
  • Mary Ann Foglio
  • Division of Chemistry of Natural Products, CPQBA UNICAMP, Campinas, Brazil.
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Caio Haddad Franco
  • Caio Haddad Franco
  • Brazilian Biosciences National Laboratory, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, Brazil.
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Carolina Borsoi Moraes
  • Carolina Borsoi Moraes
  • Brazilian Biosciences National Laboratory, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, Brazil.
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Valéria Maia de Oliveira
  • Valéria Maia de Oliveira
  • Division of Microbial Resources, CPQBA UNICAMP, Campinas, Brazil.
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  •  Received: 16 July 2018
  •  Accepted: 24 September 2018
  •  Published: 07 October 2018

Abstract

Strains previously isolated from offshore oil reservoirs in Campos Basin (RJ, Brazil) and Potiguar Basin (RN, Brazil) were tested for production of antimicrobial compounds. Sixteen bacteria produced compounds with antimicrobial activity. The strain chosen for further study was Bacillus safensis SG-32 based on its ability to inhibit 76% of the strains tested. The bacterium was grown in Marine Broth (MB) and the hexane extract was investigated for minimal inhibitory concentration (MIC) (highest activity 0.4 mg/mL against Staphylococcus aureus), antiproliferative activity against human cancer cell lines (highest activity TGI = 69.83 µg/mL against melanoma cell lines), and antiparasitic tests (maximum activity 22.49% against Trypanosoma cruzi). Bioactive extract from B. safensis SG-32 was purified using silica column chromatography. Fractions collected were tested by bioautography in order to select the fraction with antimicrobial activity. The active molecule was identified by Nuclear Magnetic Resonance (NMR) as a diterpene of the Cyathin family. In addition to the antimicrobial and antitumor activity, Cyathin type compounds are known  to stimulate the synthesis of Nerve Growth Factor (NGF), a capacity that implicates their potential as therapeutic agents to treat neurodegenerative ailments such as Alzheimer’s or Parkinson’s disease. In conclusion, this study showed that B. safensis SG-32 produces metabolites with antibacterial, antiproliferative and antiparasitic activities, and opens perspectives for further study of its potential also for nerve protection.

 

Key words: Antimicrobial, antiproliferative, Bacillus safensis, Cyathin, nerve grow factor, drug discovery.