African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5093

Full Length Research Paper

Diversity and Symbiotic Effectiveness of Rhizobium Isolates Collected from Different Faba bean (Vicia faba) Growing Areas of North and South Gondar, Ethiopia

Zewdu Teshome Awlachew
  • Zewdu Teshome Awlachew
  • Department of Biology, College of Natural and Computational Sciences, University of Gondar, P.O. Box 196, Ethiopia.
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Desalegn Adisu Kassie
  • Desalegn Adisu Kassie
  • Department of Biology, College of Natural and Computational Sciences, University of Gondar, P.O. Box 196, Ethiopia.
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Samuel Sahile Woldemariam
  • Samuel Sahile Woldemariam
  • Department of Biology, College of Natural and Computational Sciences, University of Gondar, P.O. Box 196, Ethiopia.
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  •  Received: 04 September 2018
  •  Accepted: 29 October 2018
  •  Published: 07 December 2018

Abstract

Fifty seven Rhizobium isolates were retrieved based on infection method from different faba bean growing areas of North and South Gondar, Ethiopia. In sand culture, only 35.5% of isolates showed nodulation. Analyses of variance indicated that inoculation of isolates improved tested traits significantly (p< 0.05) at all measured investigated parameters such as shoot length, shoot dry weight, and plant total nitrogen as 11, 28 and 31.3%, respectively, over nitrogen treated plants, 2, 10 and 29.4% respectively over standard Rhizobium and 55, 82.3 and 85.7% respectively over negative control treatments. Shoot dry weight was found to be strongly positively correlated with symbiotic effectiveness (r = 0.994, P <0.01). Based on symbiotic effectiveness, 80% of the tested isolates were found to be highly effective, 13% effective and only one isolate less effective. Therefore, there is a need for detail study of effective isolates tested under field conditions and molecular characterization for better classification.

 

Key words: Authentication, Legumes, Nitrogen fixation, Rhizobium, total nitrogen.