African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5188

Full Length Research Paper

Effect of cholesterol lowering multiplex lactic acid bacteria on lipid metabolism in a hamster model

Cheng-Chih Tsai
  • Cheng-Chih Tsai
  • Department of Applied Cosmetology, Master Program of Cosmetic Science, Hung Kuang University, Taichung City 43302, Taiwan, R.O.C.
  • Google Scholar
Lan-Chun Chou
  • Lan-Chun Chou
  • Department of Food Science and Technology, Hung Kuang University, Taichung City 43302, Taiwan, R.O.C.
  • Google Scholar
Shu-E Lai
  • Shu-E Lai
  • Department of Nutrition, Chung-Kang Branch, Cheng Ching Hospital, Taichung City 40764,Taiwan, R.O.C.
  • Google Scholar
Chung-Chih Huang
  • Chung-Chih Huang
  • Department of Food Science and Technology, Hung Kuang University, Taichung City 43302, Taiwan, R.O.C.
  • Google Scholar


  •  Received: 16 August 2015
  •  Accepted: 12 February 2016
  •  Published: 28 May 2016

Abstract

This study aimed to investigate the effects of a probiotic complex product including Pediococcus, Lactobacillus, and Bifidobacteria on the lipid metabolism of hamsters fed a high-fat and high-cholesterol diet. Fifty male Syrian hamsters were assigned to five experimental groups: control, high-fat plus high-cholesterol diet (HFC), and HFC supplemented with low-, medium-, and high-dose of probiotic product. The hamsters in the control group were fed an AIN-76 basal diet. A high-cholesterol diet was based on the AIN-76 basal diet, supplemented with 0.5% (w/w) cholesterol, 12% corn oil, and 3% (w/w) lard to adjust the fat content. After a one-week adaptation period, the experimental period started, during which the animals were fed for 10 weeks, and food intake and body weight were recorded periodically. Blood samples were obtained for the analysis of serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), and thiobarbituric acid reactive substances (TBARS). The results show that feeding animals with the low, medium, and high doses of probiotic complex product had significantly lowered serum LDL-C, and TBARS levels, as well as LDL-C/HDL-C ratio (P < 0.05), while we observed a significant increase in HDL-C levels (P < 0.05). These results indicate that the probiotic complex product could reduce obesity, dyslipidemia, and lipid peroxidation.

 

Key words: Probiotics, cholesterol, LDL-C/HDL-C ratio, thiobarbituric acid reactive substances, hamster.