Abstract

To investigating microbial abundance and community in confluence region of Huang, Wei and Luo rivers in Guanzhong plain in central China, plates culture were used to visualize quantity of soil microorganism colony, polymerase chain reaction and Denaturing Gradient Gel Electrophoresis (DGGE) were used to detect microorganism abundance and community. The results show that microorganism reduce in winter with the temperature lowering but increase in summer. River water can protect microorganism in sediment to repress quantity of microorganism reduce in winter. Microorganism is more abundance in vegetation surface than bench and sediment. In different three types of wetland microorganism species are also different, but proteobacteria is always a dominant bacteria in any time and any wetland. From these finding we can conclude that vegetation and soil organic matter affect the heterogeneity of microbial distribution. The presence of vegetation improves the soil alkaline environment. Microbiological composition in this area will provide a basis for construction of wetlands ecosystems.

Key words: wetland, bacteria, microorganism, microbial community.