African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Genetic characterization of Fusarium oxysporum isolated from guava in northern India

  Rupesh Kumar Mishra1,2*, B. K. Pandey2, Vijai Singh2,3*, Neelam Pathak1 and Mohd. Zeeshan1
  1Department of Biotechnology, Integral University, Lucknow-226026, India. 2Molecular Plant Pathology Laboratory, Central Institute for Subtropical Horticulture, Rehmankhera, Lucknow-227101, India. 3Division of Biotechnology and Bioinformatics, Biobrainz Life Sciences, Vikas Nagar, Lucknow- 226 022, India.
Email: [email protected]

  •  Accepted: 26 July 2013
  •  Published: 16 August 2013



Fusarium oxysporum f. sp. psidii is a causal agent of wilt disease of guava in subtropical regions globally. The production of guava fruit is affected by wilt disease thus, there is a need to develop molecular markers for accurate, rapid, specific and sensitive diagnosis ofF. oxysporum. A set of species-specific primers were developed for amplification of the internal transcribed spacer region. Subsequently, polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) of 18S-28S rRNA was used to analyse the genetic diversity and phylogenetic relationships of F. oxysporum which was isolated from northern agro-ecological regions of India. These finding provide an insight for earlier molecular diagnostic of F. oxysporum for better management of guava wilt disease in India.


Key words: Diagnostic, Fusarium oxysporum f. sp. psidii, management, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), Wilt.