African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5177

Full Length Research Paper

Degradation kinetics of resorcinol by Enterobacter cloacae isolate

  Marissa R. Jablonski1, Shraddha Shaligram2, Asifa Qureshi2*, Hemant Purohit2 and John R. Reisel3  
  1Department of Civil Engineering and Mechanics, University of Wisconsin, Milwaukee, 3200 N. Cramer St., Milwaukee, WI 53211, USA. 2Environmental Genomics Division (EGD), National Environmental Engineering Research Institute (NEERI), Nagpur India, Nehru Marg, Nagpur, 440020, India. 3Department of Mechanical Engineering, University of Wisconsin, Milwaukee, 3200 N. Cramer St., Milwaukee, WI 53211, USA.
Email: [email protected]

  •  Accepted: 17 June 2013
  •  Published: 12 July 2013

Abstract

 

Resorcinol was utilized as the sole carbon and energy source by Enterobacter cloacae(identification by 16S rDNA nucleotide sequencing Genbank Accession Number JN093148). The different concentration of resorcinol utilized by the bacterial isolate ranged between 55 and 220 mg l-1 at 30°C and pH of 7.0. It was observed that the batch experimental results were best fitted for Michaelis-Menten and Monod models (for 220 mg l-1 resorcinol) with time under defined conditions. The kinetics constants for the Michaelis-Menten equation (enzyme kinetics) were Km = 11.00 mM and Vmax = 0.03 mM min-1 and for the Monod equation (growth kinetics) was μmax = 0.0371 h-1 in the inhibitory region and KS = 22.09 mg l-1. It was assumed that enzyme reactions limit biomass production (Monod kinetics) during resorcinol degradation by E. cloacae. The enzyme kinetic model (Michaelis-Menten) used was fit to the resorcinol degradation profiles with a set of model parameters such as using pre-induced E. cloacae cells on 220 mg l-1 resorcinol.

 

Key words: Resorcinol, Michaelis-Menten, Monod, aerobic, Enterobacter cloacae.