African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4881

Full Length Research Paper

Comparison of Brucella agar, CITA and Farrell media for selective isolation of Brucella abortus from semen of bovine bulls

Gerado de Nardi Junior
  • Gerado de Nardi Junior
  • Tecnologia em Agronegocio da Faculdade de Tecnologia de Botucatu, SP, Brazil
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Jane Megid
  • Jane Megid
  • Department of Veterinary Hygiene and Public Health ? School of Veterinary Medicine and Animal Sciences ? Sao Paulo State University (UNESP), Botucatu, SP, Brazil
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Acacia Ferreira Vicente
  • Acacia Ferreira Vicente
  • Department of Veterinary Hygiene and Public Health ? School of Veterinary Medicine and Animal Sciences ? Sao Paulo State University (UNESP), Botucatu, SP, Brazil
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Fernando Jose Paganini Listoni
  • Fernando Jose Paganini Listoni
  • Department of Veterinary Hygiene and Public Health ? School of Veterinary Medicine and Animal Sciences ? Sao Paulo State University (UNESP), Botucatu, SP, Brazil
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Fabio Morato Monteiro
  • Fabio Morato Monteiro
  • Agencia Paulista de Tecnologia em Agronegocio (APTA), Bovinos de Corte, Instituto de Zootecnia, Sertaozinho-SP, Brazil
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Gustavo Henrique Batista Lara
  • Gustavo Henrique Batista Lara
  • Department of Veterinary Hygiene and Public Health ? School of Veterinary Medicine and Animal Sciences ? Sao Paulo State University (UNESP), Botucatu, SP, Brazil
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Rodrigo Garcia Motta
  • Rodrigo Garcia Motta
  • Department of Veterinary Hygiene and Public Health ? School of Veterinary Medicine and Animal Sciences ? Sao Paulo State University (UNESP), Botucatu, SP, Brazil
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Marcelo George Mungai Chacur
  • Marcelo George Mungai Chacur
  • Reproducao Animal, Universidade do Oeste Paulista (UNOESTE) ? Presidente Prudente, SP, Brazil
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Marcio Garcia Ribeiro*
  • Marcio Garcia Ribeiro*
  • Department of Veterinary Hygiene and Public Health ? School of Veterinary Medicine and Animal Sciences ? Sao Paulo State University (UNESP), Botucatu, SP, Brazil
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  •  Received: 05 November 2014
  •  Accepted: 09 February 2015
  •  Published: 04 March 2015

Abstract

Brucellosis remains as a public health concern worldwide. In domestic animals, the disease is characterized by reproductive disorders in male and female. Besides extensive use of serological tests and recent development of molecular biology techniques, microbiological culture of Brucella species is yet considered a “gold standard” method for diagnosis. Here, semen of 335 bovine bulls was subjected simultaneously to microbiological culture in Brucella agar, Farrell media, and CITA media to evaluate comparatively the best selective media for isolation of Brucella sp. Among all 335 samples, B. abortus B19 strain was isolated from semen of five (1.49%) bulls using the three selective media. However, Farrell media was considered the best selective media for microbiological diagnosis, because of allowed isolation of B. abortus B19 strain from bull semen without bacterial commensal or fungal contamination of plates.

Key words: Brucella agar, Farrell media, CITA media, bovine bulls, semen.