African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5146

Full Length Research Paper

Characterization and purification of bacteriocin produced by Enterococcus sp. GHB26 isolated from Algerian paste of dates "Ghars"

Amel Kout*
  • Amel Kout*
  • Laboratory of Biology of Microorganisms and Biotechnology, University of Oran 1 Ahmed Ben Bella, B.P. 1524, El M’Naouer, 31000 Oran, Algeria.
  • Google Scholar
Fatiha Dalache
  • Fatiha Dalache
  • Laboratory of Biology of Microorganisms and Biotechnology, University of Oran 1 Ahmed Ben Bella, B.P. 1524, El M’Naouer, 31000 Oran, Algeria.
  • Google Scholar
Halima Zadi-Karam
  • Halima Zadi-Karam
  • Laboratory of Biology of Microorganisms and Biotechnology, University of Oran 1 Ahmed Ben Bella, B.P. 1524, El M’Naouer, 31000 Oran, Algeria.
  • Google Scholar
Nour-Eddine Karam
  • Nour-Eddine Karam
  • Laboratory of Biology of Microorganisms and Biotechnology, University of Oran 1 Ahmed Ben Bella, B.P. 1524, El M’Naouer, 31000 Oran, Algeria.
  • Google Scholar


  •  Received: 29 April 2016
  •  Accepted: 06 June 2016
  •  Published: 07 July 2016

Abstract

Strain Enterococcus sp. GHB26, isolated from the Algerian paste of dates "Ghars", produced a bacteriocin. This bacteriocin was inactivated by proteolytic enzymes. Antibacterial activity of the bacteriocin was heat stable at 120°C for 20 min (533 AU/ml), stable at pH range of 2 to 12 and resistant to chemicals (SDS, EDTA, NaCl, Tween 80, Urea). The active bacteriocin from the cell-free supernatant of Enterococcus sp. GHB26 was purified by precipitation with ammonium sulfate followed by various combinations of gel filtration on a Sephadex G-25 column, cation exchange chromatography on a CM- Sephadex Cellulose column and reverse phase-high performance liquid chromatography on a C18 column. The bacteriocin was eluted as a single peak on the chromatogram from reverse phase-high performance liquid chromatography attesting the purity of this bacteriocin. The bacteriocin exhibited a bactericidal mode of action. Sodium dodecyl sulphate - polyacrylamide gel electrophoresis indicated that molecular weight of this bacteriocin is close to 3.5 kDa.

Key words: Paste of dates, bacteriocin, antibacterial activity, chromatography, mode of action.