African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5131

Full Length Research Paper

Probiotic properties of Brevibacillus brevis and its influence on sea bass (Dicentrarchus labrax) larval rearing

Abdelkarim Mahdhi1*, Fathi Kamoun2, Concetta Messina3,4, Andrea Santulli3,4 and Amina Bakhrouf1
1Laboratory of Analysis, Treatment and Valorization of Pollutants of the Environment and Products (LATVPEP), Faculty of Pharmacy, University of Monastir, 5000. Tunisia. 2National Institute of the Sciences and Technologies of the Sea (INSTM), Monastir, 5000. Tunisia. 3Marine Biochemistry Laboratory, Section of Biochemical Science, Department of BIONEC, University of Palermo, Via Barlotta 4, 91100, Trapani, Italy. 4Marine Biology Institute, Consorzio Universitario della Provincia di Trapani, Via Barlotta 4, 91100, Trapani, Italy.
Email: [email protected]

  •  Accepted: 13 July 2012
  •  Published: 13 September 2012

Abstract

Efficacy of Brevibacillus brevis strain and its influence on larval rearing of sea bass (Dicentrarchus labrax) were investigated in the present work. Biochemical analyses permit to identify this strain as B. brevis. This bacterium has an inhibitory effect against fish pathogenic bacteria, especially the genus of Vibrio. Enzymatic characterization revealed that B. brevis was lipase positive, amylase, lecithinase and caseinase negative. Adherence assays to abiotic surfaces and challenge test with Artemia larvae demonstrate that B. brevis was fairly adherent and play an important role in the enhancement of the protection of Artemia culture against pathogens. Treatment of sea bass larvae with B. brevis not affects survival compared to control group. Specific growth and linear rate of sea bass larvae feed by Artemia enriched with potential probiont was improved compared to the control treatment. Therefore, this investigation suggested that the tested strain can be considered as promising potential probiotic that can be used incorporated in Artemia nauplii for rearing of fish and selfish larvae based on the enhancement of survival and growth rate and pathogens elimination of in vivo and in vitro conditions.

 

Key words: Brevibacillus brevis, probiotics, antagonism, adhesion, Artemia, Sea bass larvae, aquaculture.