African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5045

Full Length Research Paper

Seroprevalence of avian origin H3N2 canine influenza virus infection in pet dogs in Shenzhen, China

Fu-Rong Zhao1,2#, Shou-Jun Li1#, Dong-Hui Zhou2, Ning Chen3,  Yan-Zhong Zhang4, Wen-Bao Qi1, Pei-Rong Jiao1, Ming Liao1, Guang-Zhi Tong5, and Gui-Hong Zhang1*
  1College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People’s Republic of China. 2State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu Province 730046, People’s Republic of China. 3Shenzhen Institute for Drug Control, Shenzhen, Guangdong Province 518057, PR China. 4Shenzhen Rui-Peng Pet Hospitol, Shenzhen, Guangdong Province 518001, PR China. 5Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute, CAAS Shanghai 200241,People’s Republic of China.
Email: [email protected]

  •  Accepted: 22 November 2011
  •  Published: 30 December 2011

Abstract

 

Canine influenza virus (CIV) is an emerging pathogen that causes severe and acute respiratory disease in dogs. Canine influenza is caused by two subtypes of influenza. A virus: H3N2 and H3N8. In recent years, surveys of avian origin CIV infection in dogs have been reported worldwide. However, little is known about the prevalence of CIV in pet dogs in China. In the present study, the prevalence of avian origin CIV H3N2 in pet dogs in Shenzhen, Southern China was investigated using the enzyme-linked immunosorbent assay (ELISA) and hemagglutination inhibition (HI) assay.Thirty-one (6.71%) of the 462 serum samples tested were seropositive for avian origin CIV by ELISA. Use of the HI test revealed the presence of anti-H3 antibodies in 28 (6.06%) of 462 serum samples. The prevalence ranged from 4.87% (HI) or 6.19% (ELISA) to 7.41% among dogs of different ages, with high prevalence in pet dogs of 1 to -3 years old, but low prevalence in pet dogs≤1 year. The seroprevalence in female dogs was 5.21%, and in male dogs it was 7.78% (ELISA) or 6.67% (HI). These findings demonstrated that avian origin canine influenza virus infection is prevalent in pet dogs and can spread rapidly through local dog populations, which indicates its potential for becoming established in pet dogs throughout China.

 

Key words: Canine influenza virus, seropervalence, pet dog, enzyme-linked immunosorbent assay (ELISA), hemagglutination inhibition (HI) assay.