African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5182

Full Length Research Paper

Biosynthesis, optimization, purification and characterization of gold nanoparticles

Shivaji S. Waghmare
  • Shivaji S. Waghmare
  • Department of Microbiology, Fergusson College, Pune 411 004. (M.S.) India
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Arvind M. Deshmukh
  • Arvind M. Deshmukh
  • Department of Microbiology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, subcenter, Osmanabad, 413 501. (M.S.) India
  • Google Scholar
Zygmunt Sadowski
  • Zygmunt Sadowski
  • Department of Chemical Engineering, Wroclaw University of Technology, Wybrzeze Wyspianskiego, 27 Wroclaw, Poland
  • Google Scholar

  •  Received: 26 March 2010
  •  Accepted: 05 July 2013
  •  Published: 08 January 2014


Many microorganisms produce intracellular metal nanoparticles. Aqueous gold, when exposed to several actinomycetal strains, become thereby, leading to the formation of gold nanoparticles. The use of microorganisms in the synthesis of nanoparticles is emerging as an eco-friendly and exciting approach gold for recovery. Streptomyces hygroscopicus was used for the biosynthesis of gold nanoparticles. UV and visible spectroscopic studies of biofilms revealed better synthesis of nanoparticles. It was observed that better biosynthesis of gold nanoparticles occurred when cell biomass treated with 10-3 and 10-4 mM HAuCl4 solution as compared to other dilutions. The pH 7.0 was found to be optimum for the biosynthesis of gold nanoparticles. The TEM study revealS that there was evidence of gold nanoparticles synthesized by S. hygroscopicus. The results demonstrate that spherical gold nanoparticles in the range of 10 to 20 nm were observed at pH value of 7.0. The actinomycetal biomass and various concentration of aqueous HAuCl4 solution were incubated, it was found that 10-4 concentration shows excellent colour of the actinomycetal biomass.


Key words: Biosynthesis, optimization, gold nanoparticles, purification, characterization.