African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5123

Full Length Research Paper

Molecular characterization of carbapenem resistant Gram-negative rods in Neonatal Intensive Care Unit of Mansoura University Children's Hospital

Heba Elsayed Eldegla
  • Heba Elsayed Eldegla
  • Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
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Islam Nour
  • Islam Nour
  • Neonatal Intensive Care Unit, Mansoura University Children’s Hospital, Mansoura, Egypt.
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Nehad Nasef
  • Nehad Nasef
  • Neonatal Intensive Care Unit, Mansoura University Children’s Hospital, Mansoura, Egypt.
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Basma Shouman
  • Basma Shouman
  • Neonatal Intensive Care Unit, Mansoura University Children’s Hospital, Mansoura, Egypt.
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Hesham Abdel-Hady
  • Hesham Abdel-Hady
  • Neonatal Intensive Care Unit, Mansoura University Children’s Hospital, Mansoura, Egypt.
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Mayada Zeid
  • Mayada Zeid
  • Departement of Pediatrics, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
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Nawal S. Gouda
  • Nawal S. Gouda
  • Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
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Manal M. Nomir
  • Manal M. Nomir
  • Clinical Pathology, Mansoura University Student Hospital, Mansoura, Egypt.
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Abd Elazeez Shabaan
  • Abd Elazeez Shabaan
  • Neonatal Intensive Care Unit, Mansoura University Children’s Hospital, Mansoura, Egypt.
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Noha Mostafa Mahmoud
  • Noha Mostafa Mahmoud
  • Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
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  •  Received: 07 February 2021
  •  Accepted: 13 May 2021
  •  Published: 30 June 2021

Abstract

Carbapenems are group of extended-spectrum β-lactam antimicrobials frequently used for treating multidrug-resistant Gram-negative bacilli (GNB) infections. This study aimed at detecting and characterizing carbapenem resistance (CR) genes among GNB isolated from patients treated in neonatal intensive care unit (NICU) of Mansoura University Children's Hospital (MUCH), Egypt. It is a prospective study conducted from 2015 to 2016. A total of 158 GNB isolates were examined for CR both phenotypically and genotypically. Among 158 Gram negative isolates, there were 58 (36.7%) CR strains. Extended-spectrum β-lactamase (ESBL) production was confirmed in all 58 (100%) isolates. Carbapenemase production was detected in 52 (89.5%) strains while metallo beta-lactamase (MBL) production was found in 33 (56.9%) strains.  Molecular characterization of CR strains revealed that 57 (98.3%) isolates were positive for carbapenemase encoding genes. KPC gene was the most frequent detected gene (34/58).  VIM, IPM, OXA and NDM genes were also detected in 15, 13, 9 and 1 isolate, respectively. Only one isolate was negative for all encoding resistance genes despite positive for ESBL phenotype. Infection with CR strains has been increasing in clinical settings which limit the use of carbapenems.

 

Key words: Gram-negative bacilli, carbapenem resistance, carbapenemase, metallo beta-lactamase, multiplex polymerase chain reaction (PCR), carbapenemase encoding genes, neonatal intensive care unit.