Four bacterial associates were isolated from the Arctic lichen Stereocaulon sp. and identified based on their complete 16S rRNA gene sequences. Cell cultures were extracted using different solvents and tested for their antimicrobial properties using paper disk diffusion assay and MIC test against six test bacteria. Results show that the ethyl acetate of bacterial isolate is a most effective solvent to extract metabolites from the bacterial associates. Bosea vestrissi 34635T (KOPRI 26642) of four bacterial associates showed highest activity as shown as inhibition zone was 7 to 14, and MIC value was 52.7 to >1000 against six test bacteria.
Key words: Lichen, Stereocaulon sp., bacterial associate, antimicrobial activity, MIC test.
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