African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5093

Full Length Research Paper

Methicillin-resistant Staphylococcus spp. in the nasal cavity of dental surgeon’s professors

Camila Fonseca Alvarenga
  • Camila Fonseca Alvarenga
  • Programa de Pós-Graduação em Ciências da Saúde, Faculdade de Medicina, Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brazil.
  • Google Scholar
Késia Cristina de Oliveira Batista
  • Késia Cristina de Oliveira Batista
  • Programa de Pós-Graduação em Enfermagem, Faculdade de Enfermagem, UFG, Goiânia, Goiás, Brazil.
  • Google Scholar
Késia Cristina de Oliveira Batista
  • Késia Cristina de Oliveira Batista
  • Instituto de Patologia Tropical e Saúde Pública, UFG, Goiânia, Goiás, Brazil.
  • Google Scholar
Lara Stefânia Netto de Oliveira Leão Vasconcelos
  • Lara Stefânia Netto de Oliveira Leão Vasconcelos
  • Instituto de Patologia Tropical e Saúde Pública, UFG, Goiânia, Goiás, Brazil.
  • Google Scholar
Juliana Lamaro Cardoso
  • Juliana Lamaro Cardoso
  • Instituto de Patologia Tropical e Saúde Pública, UFG, Goiânia, Goiás, Brazil.
  • Google Scholar
Maria Cláudia Porfirio André
  • Maria Cláudia Porfirio André
  • Instituto de Patologia Tropical e Saúde Pública, UFG, Goiânia, Goiás, Brazil.
  • Google Scholar
Dayane de Melo Costa
  • Dayane de Melo Costa
  • Programa de Pós-Graduação em Enfermagem, Faculdade de Enfermagem, UFG, Goiânia, Goiás, Brazil.
  • Google Scholar
Enilza Maria Mendonça de Paiva
  • Enilza Maria Mendonça de Paiva
  • Faculdade de Odontologia, UFG, Goiânia, Goiás, Brazil.
  • Google Scholar
Anaclara Ferreira Veiga Tipple
  • Anaclara Ferreira Veiga Tipple
  • Programa de Pós-Graduação em Ciências da Saúde, Faculdade de Medicina, Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brazil.
  • Google Scholar


  •  Received: 07 June 2019
  •  Accepted: 11 July 2019
  •  Published: 31 July 2019

Abstract

The aim of the study is to investigate the methicillin-resistant Staphylococcus spp. nasal colonization among dental surgeon professors. Dental surgeon professors of a Higher Education Institution (HEI) responded to a questionnaire covering sociodemographic, employment and behavioral data, and were subjected to clinical specimen collection by nasal swab. Identification and susceptibility testing of bacteria were performed by automated method (Vitek 2 compactTM). Susceptibility to mupirocin was tested by disk-diffusion method. The detection of mecA and lukS-F genes was performed by PCR. The genetic similarity among the isolates was determined by Pulsed Field Gel Electrophoresis. Four (9.7%) dental surgeon professors were colonized by methicillin-resistant Staphylococcus spp. and claim have provided care to patients without wearing surgical masks (1/4) and/or gloves (4/4), and had the habit of keeping surgical masks on the chin (1/4). Two S. aureus and one S. epidermidis isolates were mecA gene positives. MLSB complex (inducible), mupirocin and sulfamethoxazole/trimethoprim resistance were also detected. The lukS-F gene was not detected in any S. aureus and no genetic similarity was found among the isolates. Dental surgeon professors were found to be colonized with methicillin-resistant Staphylococcus spp. and declared noncompliance to infection control practices, posing risk of infection to themselves, patients, students and their families.

 

Key words: Occupational dentistry, antimicrobials/antimicrobial resistance, dental education, infection control, bacteria, infectious disease(s).