African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4989

Full Length Research Paper

Detection of common resistance genes of Gram-negative bacteria by DNA microarray assay

Ting Cai*, Shun Zhang, Qiao-yun Li, Chun-xiu Zhang and Yan-zi Chang        
Ningbo No.2 Hospital, Ningbo, Zhejiang 315010, China.
Email: [email protected]

  •  Accepted: 21 October 2011
  •  Published: 16 January 2012

Abstract

To design a DNA microarray for the detection of common resistance genes of Gram-negative bacteria, we collected 70 strains of Gram-negative bacilli (Klebsiella pneumoniae,Escherichia coliEnterobacter cloacaePseudomonas aeruginosaAcinetobacter baumannii), and the primers and probes were designed and synthesized according to the known resistance genes to prepare the deoxyribonucleic acid (DNA) microarray for the detection of these resistance genes. Then, hybridization and subsequent scanning were performed. The results of DNA microarray assay were compared with results from the polymerase chain reaction (PCR). Results showed that the consistency rate was 100.00% in the detection of 8 resistance genes (TEM, SHV, CTX-M, DHA, CIT, VIM, KPC and OXA-23). PCR assay revealed the resistance gene IMP in 1 strain of P. aeruginosa, which was absent in the DNA microarray assay. The DNA microarray designed in the present study has clinical value in the detection of common resistance genes of Gram-negative bacteria. 

 

Key words: Gram-negative bacteria, resistance gene, DNA microarray.

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