African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5165

Full Length Research Paper

Candida spp. associated with hot beverages of coffee and tea sold on street in Côte d’Ivoire

Atobla Koua
  • Atobla Koua
  • Laboratory of Biotechnologies, Department of Biosciences, Felix Houphouet-Boigny University, 22 BP 582 Abidjan 22, Côte d´Ivoire.
  • Google Scholar
Blé Yatanan Casimir
  • Blé Yatanan Casimir
  • Department of Agronomic Forest and Environmental Engineering, University of Man, BP 20 Man, Côte d’Ivoire.
  • Google Scholar
Ouattara Mohamed Baguy
  • Ouattara Mohamed Baguy
  • Department of Bacteriology and Virology, National Reference Center for Antibiotics, Pasteur Institute, Abidjan, 01 BP 490 Abidjan 01, Côte d’Ivoire.
  • Google Scholar
Touré Naka
  • Touré Naka
  • Laboratory of Biochemical and Microbiology of Biology Sciences of Korhogo, Peleforo Gon Coulibaly University, BP 1328 Korhogo, Côte d'Ivoire.
  • Google Scholar
Adjehi Dadié
  • Adjehi Dadié
  • Department of Food Sciences and Technologies (STA), Laboratory of Microbiology and Biotechnology, Nangui Abrogoua University, 02 BP 801 Abidjan 02, Côte d'Ivoire.
  • Google Scholar
Niamké Sébastien
  • Niamké Sébastien
  • Laboratory of Biotechnologies, Department of Biosciences, Felix Houphouet-Boigny University, 22 BP 582 Abidjan 22, Côte d´Ivoire.
  • Google Scholar


  •  Received: 13 June 2021
  •  Accepted: 27 July 2021
  •  Published: 30 September 2021

Abstract

Some Candida species are considered as human opportunistic pathogens and can play an important role in spontaneous fermentations, but also as beverage-spoiling microorganisms. The aim of this pioneering study was to investigate Candida spp. in hot beverages, which is consumed mostly on the streets in Côte d’Ivoire. Yeast strains were isolated from 400 hot beverages of tea (200) and coffee (200) samples. Yeast cultures were identified at genus and species level by MALDI-TOF mass spectrometry at the biobank laboratory of the Pasteur Institute of Côte d’Ivoire. A total of 37 Candida isolates were clearly identified by MALDI-TOF (MS) and revealed 11 species of Candida: C. krusei (21.6%), C. tropicalis (18.9%), C. parapsilosis (16.2%), C. guilliermondii (16.2%), C. pelliculosa (8.1%), C. dubliniensis (5.4%), C. rugosa (2.7%), C. kefyr (2.7%), C. silvicola (2.7%), C. lusitaniae (2.7%) and C. orthopsilosis (2.7%). The results showed that C. krusei and C. tropicalis were the dominant yeasts in hot beverages from street vendors. Candida species were more isolated in tea (10%) than in coffee (8.5%). C. tropicalis, C. pelliculosa and C. krusei were more isolated in Cocody. C. guilliermondii and C. parapsilosis were more isolated in Port-Bouët. C. dubliniensis was only isolated in Yopougon town. The presence of Candida spp. in street hot beverages could cause a sanitary risk to consumers or be used as a novel source for biotechnological uses to be explored in future work.

 

Key words: Street hot beverages, Candida spp., coffee, tea, food safety.