African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5093

Full Length Research Paper

Polyphenolic profile, and antioxidant and antifungal activities of honey products in Benin

François Ezin Azonwade
  • François Ezin Azonwade
  • Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
  • Google Scholar
Bertin Gbaguidi
  • Bertin Gbaguidi
  • Laboratoire de Biomembrane et de Signalisation Cellulaire, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Bénin.
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Armand Paraïso
  • Armand Paraïso
  • Laboratoire de Protection des Végétaux, de Pathologie et Parasitologie des Abeilles, Faculté d’Agronomie, Université de Parakou, Bénin.
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Durand M. Dah-Nouvlessounon
  • Durand M. Dah-Nouvlessounon
  • Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
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Elvire Goubalan
  • Elvire Goubalan
  • Laboratoire de Physicochimie et d’Evaluation Sensorielle des Aliments, Faculté des Sciences Agronomiques, Université d’Abomey-Calavi, Bénin.
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Farid Baba-Moussa
  • Farid Baba-Moussa
  • Laboratoire de Microbiologie et de Technologie Alimentaire, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, Benin.
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Madjid Amoussa
  • Madjid Amoussa
  • Unité de Biochimie des Substances Naturelles et Bioactives, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, Bénin.
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Latifou Lagnika
  • Latifou Lagnika
  • Unité de Biochimie des Substances Naturelles et Bioactives, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, Bénin.
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Victorien Dougnon
  • Victorien Dougnon
  • Laboratoire de Recherche en Biologie Appliquée, Ecole Polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, Cotonou, Bénin.
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Lamine Baba-Moussa
  • Lamine Baba-Moussa
  • Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
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  •  Received: 29 October 2017
  •  Accepted: 30 November 2017
  •  Published: 07 January 2018

Abstract

The aim of this study was to characterize polyphenolic compounds, antifungal and antioxidant activities of polyfloral honey collected from three different phytogeographical zones in Benin duing dry and rainy seasons. Spectrophotometrically at 517 nm, DPPH scavenging activity of tested samples was measured, while antifungal activity, total polyphenols, flavonoids and condensed tannins were evaluted by Dohou, Singleton and Zhishen methods, and a slightly modified method of Sun, respectively. Results revealed that only season has significant influence (P <0.05) on the flavonoids content and total phenols. The highest total phenols (781 ± 46 µgGAE/gs) and flavonoids (528 ± 31 µgCE/g) contents were obtained respectively from honey samples of the rainy and dry seasons. The samples collected in the rainy season in the three zones, have better antifungal activity than those collected in the dry season. The lowest IC50 recorded was 27.58 ± 4.44 μg.µl-1 which showed that the highest antioxidant activity can be found in honey samples of the rainy season collected from the Sudanian zone. Overall, this study confirms that all investigated honey samples were good sources of polyphenolic compounds, and exhibit antifungal and antioxidant actvities.
 
Keywords: Antifungal, antiioxidant, honey, Benin.