African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4998

Full Length Research Paper

Evaluation of antibacterial activity of some non-steroidal anti-inflammatory drugs against Escherichia coli causing urinary tract infection

Eman Farouk Ahmed
  • Eman Farouk Ahmed
  • Department of Microbiology and Immunology, Faculty of Pharmacy, Deraya University, Minia, Egypt.
  • Google Scholar
Rehab Mahmoud Abd El-Baky
  • Rehab Mahmoud Abd El-Baky
  • Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia, Egypt.
  • Google Scholar
Abo Bakr F Ahmed
  • Abo Bakr F Ahmed
  • Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia, Egypt.
  • Google Scholar
Nancy Gameel Fawzy
  • Nancy Gameel Fawzy
  • Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia, Egypt.
  • Google Scholar
Neveen Abdel Aziz
  • Neveen Abdel Aziz
  • Department of Microbiology and Immunology, Faculty of Pharmacy, Ahram Canadian University, 12451- Sixth of October City, Egypt.
  • Google Scholar
Gamal Fadl Mahmoud Gad
  • Gamal Fadl Mahmoud Gad
  • Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia, Egypt.
  • Google Scholar


  •  Received: 23 June 2016
  •  Accepted: 19 August 2016
  •  Published: 14 September 2016

Abstract

Extensive use of antibiotics for urinary tract infections has led to the emergence of drug-resistant microorganisms and one solution to this problem is to search for non-antibiotic compounds that exert anti-bacterial activity through different mechanisms such as non-steroidal anti-inflammatory drugs (NSAIDs). In this study, out of 100 urine samples; 48 Escherichia coli strains were detected, 47.9% were multi-drug resistant. The antibiogram resistance pattern of the strains was carried out by agar dilution method. Diclofenac sodium, indomethacin, aspirin and ibuprofen were tested against the E. coli isolates. Diclofenac sodium showed the lowest MIC50 and MIC90; 8 and 256 mg/ml, respectively. Aspirin showed MIC50 of 64 mg/ml, while both indomethacin and ibuprofen showed MIC50 of 256 mg/ml. Indomethacin, aspirin and ibuprofen showed the same MIC90 of 1024 mg/ml. The combined effects of the four NSAIDs and five antibiotics (Amoxicillin, Augmentin, Cefotaxime, Ciprofloxacin and Gentamicin) were tested on five resistant clinical E. coli strains by checkerboard dilution technique. All the tested NSAIDs significantly reduced the minimum inhibitory concentrations (MICs) of antibiotics against the tested bacteria and fractional inhibitory concentration indices (FICIs) for this combination ranged from 0.03 to 0.5. In this study, leakage of intracellular components suggests that the effect of NSAIDs on E. coli could be the formation of pores in the plasma membrane and scanning electron microscopy (SEM) observations confirmed the damage to the structural integrity of the tested bacteria. In conclusion, NSAIDs showed antibacterial activity against E. coli causing urinary tract infections (UTIs), a combination of them and antibiotics exhibited good synergism and the mechanism of their action was by damaging the bacterial cell membrane.

 

Key words: Urinary tract infection (UTI), Escherichia coli, NSAIDs, antibacterial resistance, antibacterial activity, synergism.