African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Short Communication

Dual-path platform (DPP) and enzyme-linked immunosorbent assay (ELISA): Change the sequence of the tests does not change the number of positive dogs for canine visceral leishmaniasis

Sara Santos Almeida
  • Sara Santos Almeida
  • Laboratório de Biomoléculas e Vacinas, Gurupi-TO, Universidade Federal do Tocantins – UFT, Brasil.
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Carla Lobo Gomes
  • Carla Lobo Gomes
  • Laboratório de Biomoléculas e Vacinas, Gurupi-TO, Universidade Federal do Tocantins – UFT, Brasil.
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Elaynne Costa Silva
  • Elaynne Costa Silva
  • Laboratório de Biomoléculas e Vacinas, Gurupi-TO, Universidade Federal do Tocantins – UFT, Brasil.
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Sarah Tolentino Rocha Brandao
  • Sarah Tolentino Rocha Brandao
  • Laboratório de Biomoléculas e Vacinas, Gurupi-TO, Universidade Federal do Tocantins – UFT, Brasil.
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Wellida Patricia Aviz
  • Wellida Patricia Aviz
  • Laboratório de Biomoléculas e Vacinas, Gurupi-TO, Universidade Federal do Tocantins – UFT, Brasil.
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Larissa Pinheiro
  • Larissa Pinheiro
  • Laboratório de Biomoléculas e Vacinas, Gurupi-TO, Universidade Federal do Tocantins – UFT, Brasil.
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Mauricio Oviedo Paciello
  • Mauricio Oviedo Paciello
  • Laboratório de Biomoléculas e Vacinas, Gurupi-TO, Universidade Federal do Tocantins – UFT, Brasil.
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Alex Sander Rodrigues Cangussu
  • Alex Sander Rodrigues Cangussu
  • Laboratório de Biomoléculas e Vacinas, Gurupi-TO, Universidade Federal do Tocantins – UFT, Brasil.
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Raimundo Wagner de Souza Aguiar
  • Raimundo Wagner de Souza Aguiar
  • Laboratório de Biomoléculas e Vacinas, Gurupi-TO, Universidade Federal do Tocantins – UFT, Brasil.
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Luiz Carlos Bertucci Barbosa
  • Luiz Carlos Bertucci Barbosa
  • Universidade Federal de Itajubá (UNIFEI), Minas Gerais, Brazil
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Rodolfo Cordeiro Giunchetti2
  • Rodolfo Cordeiro Giunchetti2
  • Laboratório de Biologia das Interações Celulares, Universidade Federal de Minas Gerais – UFMG, Brasil.
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Kelvinson Fernandes Viana*
  • Kelvinson Fernandes Viana*
  • Laboratório de Biologia das Interações Celulares, Universidade Federal de Minas Gerais – UFMG, Brasil.
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  •  Received: 09 September 2016
  •  Accepted: 06 December 2016
  •  Published: 21 January 2017

Abstract

The Brazilian Ministry of Health determined in 2012 that the official protocol for diagnosis of Canine Visceral Leishmaniasis (CVL) would be the Dual-Path Platform (DPP) for screening, followed by enzyme-linked immunosorbent assay (ELISA) for confirmation. This study evaluated serum samples from 426 dogs from a region in northern Brazil. All samples were tested according to the Official Protocol and the sequence inverting (ELISA followed DPP). Regardless of the protocol adopted, prevalence (14.7%) has not changed. The approach using ELISA followed by DPP state that, the number of positive animals in screening was higher compared to the official protocol. Screen the ELISA test could be more appropriate.

Key words: Canine visceral leishmaniasis, Dual-Path Platform (DPP), enzyme-linked immunosorbent assay (ELISA), tocantins.