African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5238

Full Length Research Paper

An evaluation of the effect of graphene oxide on Saccharomyces cerevisiae

Nafisat Omotayo
  • Nafisat Omotayo
  • Department of Biology, College of Science, Engineering and Technology, Texas Southern University, USA.
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Onyanobi Abel-Anyebe
  • Onyanobi Abel-Anyebe
  • Center for Biological and Environmental Science Research, College of Science Engineering and Technology, Texas Southern University, USA.
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Oluwasogo Olalubi
  • Oluwasogo Olalubi
  • Department of Public Health, School of Basic Medical Sciences, Kwara State University, Kwara State, Nigeria.
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Obaid Ullah
  • Obaid Ullah
  • Center for Biological and Environmental Science Research, College of Science Engineering and Technology, Texas Southern University, USA.
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Shahlla Mir
  • Shahlla Mir
  • Department of Biology, College of Science, Engineering and Technology, Texas Southern University, USA.
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Opeyemi Ojekunle
  • Opeyemi Ojekunle
  • Department of Environmental and Interdisciplinary Science, College of Science Engineering and Technology (SURP), Texas Southern University, USA.
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Adedamola Olatoregun
  • Adedamola Olatoregun
  • Department of Environmental and Interdisciplinary Science, College of Science Engineering and Technology (SURP), Texas Southern University, USA.
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Judith Anglin
  • Judith Anglin
  • Department of Interdisciplinary Health Sciences, College of Allied Health Sciences, Augusta University, Augusta, Georgia, USA.
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Ayodotun Sodipe
  • Ayodotun Sodipe
  • Department of Biology, College of Science, Engineering and Technology, Texas Southern University, USA.
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  •  Received: 23 August 2018
  •  Accepted: 14 November 2018
  •  Published: 21 December 2018

Abstract

Graphene oxide (GO) has a wide range of applications. It has been identified to have significant potential for important biomedical and biochemical uses. Graphene and its derivatives are known to elicit toxic effects. Saccharomyces cerevisiae is a versatile yeast of importance with several uses, one of which is its application as a human probiotic. The interaction of GO with living systems is of interest and relevance. The effect of GO on the yeast S.cerevisiae was investigated. The yeast cell line was cultured in a liquid media composed of Yeast Peptose Dextrose and incubated at room temperature (25°C) for 72 h. Antibody RAD 17 was labelled with fluorescent dye fluorescein isothiocyanate (FITC) to detect cell structure change. 500, 750, 1000 and 1250 μg/ml GO aliquots were prepared from a stock solution and 96 microplate wells of S. cerevisiae were treated with various doses of GO for 24, 48 and 72 h. Microscopic analysis on treated yeast showed no apparent effect on the cell after exposure to GO.

 

Key words: Graphene oxide, Saccharomyces cerevisiae, yeast cell line, cell culture, incubation, toxicity, cell wall.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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