African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5090

Full Length Research Paper

Biological activity and production of metabolites from Amazon endophytic fungi

Raiana Silveira Gurgell
  • Raiana Silveira Gurgell
  • Laboratório de Química Aplicada à Tecnologia, Escola Superior de Tecnologia, Universidade do Estado do Amazonas, Manaus, Brazil.
  • Google Scholar
Juliana Gisele Corrêa Rodrigues
  • Juliana Gisele Corrêa Rodrigues
  • Laboratório de Química Aplicada à Tecnologia, Escola Superior de Tecnologia, Universidade do Estado do Amazonas, Manaus, Brazil.
  • Google Scholar
Rosiane Rodrigues Matias
  • Rosiane Rodrigues Matias
  • Laboratório de Química Aplicada à Tecnologia, Escola Superior de Tecnologia, Universidade do Estado do Amazonas, Manaus, Brazil.
  • Google Scholar
Bárbara Nunes Batista
  • Bárbara Nunes Batista
  • Laboratório de Química Aplicada à Tecnologia, Escola Superior de Tecnologia, Universidade do Estado do Amazonas, Manaus, Brazil.
  • Google Scholar
Rafael Lopes e Oliveira
  • Rafael Lopes e Oliveira
  • Laboratório de Química Aplicada à Tecnologia, Escola Superior de Tecnologia, Universidade do Estado do Amazonas, Manaus, Brazil.
  • Google Scholar
Patrícia Melchionna Albuquerque
  • Patrícia Melchionna Albuquerque
  • Laboratório de Química Aplicada à Tecnologia, Escola Superior de Tecnologia, Universidade do Estado do Amazonas, Manaus, Brazil.
  • Google Scholar


  •  Received: 07 September 2019
  •  Accepted: 03 February 2020
  •  Published: 29 February 2020

Abstract

Obtaining bioactive metabolites from endophytic microorganisms has become increasingly more interesting in the last few decades, since endophytes are known for their biotechnological potential. However, studies involving endophytic microbiota from tropical hosts are still scarce. In this study, the production of bioactive metabolites from endophytic fungi isolated from Amazonian plants were evaluated. Two fungi (Talaromyces sp. F15 and Aspergillus sp. F18) isolated from Myrcia guianensis (Myrtaceae) and one (Penicillium sp. F3) isolated from Euterpe precatoria (Arecaceae) were analysed. The fungi were cultivated in liquid medium and their metabolites were tested for antimicrobial, antioxidant and cytotoxic activity. Amylase, cellulase and lipase production, as well as biosurfactant production, were also evaluated. The metabolites of Aspergillus sp. F18 showed 69.4% antioxidant activity against DPPH free-radical molecules and cytotoxic activity against A. salina. Penicillium sp. F3 showed cytotoxic activity and stood out as the best amylase producer (31 U/ml). Talaromyces sp. F15 was the best lipase producer (4.5 U/ml) and the best biosurfactant source, with 33.3% emulsification index. These Amazonian host-associated fungi showed biotechnological potential, which are believed should be further investigated in order to elucidate the chemical structure of the metabolites responsible for the activities described here, as well as optimize their production.

 

Key words: Enzymes, lipase, cellulase, amylase, biosurfactant, antioxidant, antimicrobial, cytotoxic.