African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4970

Full Length Research Paper

Isolation and molecular identification of polyaromatic hydrocarbons- utilizing bacteria from crude petroleum oil samples

Eman, A. H. Mohamed1,2*, Naeima M. H. Yousef 1,3 and Azza, G. Farag1,4
1Biotechnology Department, Faculty of Science, Taif University, Saudi Arabia, Kingdom of Saudi Arabia. 2Botany Department, Faculty of Science, Damanhour University, Damanhour, Egypt. 3Botany Department, Faculty of Science, Asuit University, Egypt.  4Virus and Phytoplasma Resource Department, Plant Pathology Resource Institute, ARC, Giza, Egypt.
Email: [email protected]

  •  Accepted: 03 September 2012
  •  Published: 25 December 2012


Polyaromatic hydrocarbons (PAHs) are worldwide contaminants that can reach toxic concentrations that are detrimental to the environment and human health. In this study, three different isolates (Klebsiella oxytoca, Klebsiella pneumonia and Acinetobacter sp.) from crude petroleum oil samples were found to be PAHs utilizers. They are able to utilize four different PAHs, phenanthrene, fluoranthene, pyrene, and benzene. K. oxytoca is the most efficient utilizer of the PAHs rather than K. pneumonia and Acinetobacter sp. It can utilize almost 83% of benzene after 48 h of shaking, if supplemented with nitrogen source. The isolates have been molecularly identified by partial sequencing of the 16S rDNA gene (approximately 900 bp) and the results demonstrated a high degree of homology to K. oxytoca (up to 99% similarity), K. pneumonia (from 97 to 99% similarity) andAcinetobacter sp (up to 99% similarity).


Key words: Acinetobacter, Klebsiella, PAHs, 16S rDNA.

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