African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4986

Full Length Research Paper

Shiga toxigenic, enteroinvasive and enteropathogenic Escherichia coli in fish from experimental fish farm (Layo), Côte d’Ivoire

Kouadio-Ngbesso Nadège
  • Kouadio-Ngbesso Nadège
  • Oceanology Research Center, BPV 18 Abidjan, Côte d'Ivoire.
  • Google Scholar
Kouamé-Sina Sylvie Mireille
  • Kouamé-Sina Sylvie Mireille
  • Platform of Molecular Biology, Institut Pasteur, 01 BP 490 Abidjan 01, Côte d'Ivoire.
  • Google Scholar
Koffi Ahua René
  • Koffi Ahua René
  • Ecology Research Center, Biotechnology and Microbiology Laboratory, University Nangui Abrogoua, 08 BP 109 Abidjan 08, Côte d’Ivoire.
  • Google Scholar
Toulé Aubin Cyrille
  • Toulé Aubin Cyrille
  • Biotechnology and Microbiology of Food Laboratory, University Nangui Abrogoua, Formation and Research Unity-Food and Technology Sciences, 02 BP 801 Abidjan 02, Côte d’Ivoire.
  • Google Scholar
Adingra Ama Antoinette
  • Adingra Ama Antoinette
  • Oceanology Research Center, BPV 18 Abidjan, Côte d'Ivoire.
  • Google Scholar
Dadié Adjéhi Thomas
  • Dadié Adjéhi Thomas
  • Biotechnology and Microbiology of Food Laboratory, University Nangui Abrogoua, Formation and Research Unity-Food and Technology Sciences, 02 BP 801 Abidjan 02, Côte d’Ivoire.
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  •  Received: 23 March 2019
  •  Accepted: 24 May 2019
  •  Published: 21 June 2019

Abstract

The objective of this study was to know the sanitary quality of fish coming from Layo farm. Twenty fishes (Oreochromis niloticus), were selected in four ponds and Escherichia coli were isolated in gills and viscera according to microbiological methods. One hundred and twenty strains of E. coli were isolated, and their virulence was performed by polymerase chain reaction (PCR) using specific primers. Eleven (11) strains (9.16%) including 7 strains of gills (11.66%) and 4 strains of viscera (6.66%) had virulence genes eae, Stx1, Stx2 or ial. Atypical Enteropathogenic E. coli (EPEC, eae+, lack of bfp) was isolated from gills (5%) and viscera (1.66%). Shiga toxigenic E. coli (STEC) with genes eae + Stx2, Stx1 and Stx2 were isolated in viscera (5%) and gills (3.33%). For Enteroinvasive E. coli (EIEC), ial gene was isolated in gills (3.33%) but no ipah gene. Enterotoxinogen E. coli (ETEC) with lt gene and Enteroaggregating E. coli (EAEC) with aggA gene were not detected in this study. This study revealed that some fish from Layo farm are carriers of virulent E. coli that can cause serious human diseases and can lead to consumer death if cooking is insufficient or by cross-contamination. This therefore poses a real public health problem.

 

Key words: Escherichia coli pathogens, fish, ponds, public health.